Registration Dossier

Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: 4a - Abstract

Data source

Reference
Reference Type:
publication
Title:
In vivo and in vitro Mitogenicity of 1,3-Diphenylguanidine : Analysis of DNA Synthesis and Cell Proliferation
Author:
Bempong MA
Year:
1989
Bibliographic source:
Environ Mutagen, 6, 431-432.

Materials and methods

Type of study / information:
Analysis of DNA synthesis and celle proliferation
Test guideline
Qualifier:
no guideline followed

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
No data

Results and discussion

Any other information on results incl. tables

The result of the study showed that chronic exposure to DPG stimulated pleen enlargement and that the rate of spleen enlargement exponentially commensurated with in vivo and in vitro proliferation of spleen cells. DNA synthesis, in response to DPG showed time-dependent increases similar to spleen growth and cell proliferation kinetics. Additionally, spleen cells from DPG-treated animals "modified" the medium in which they were cultured and that when spleen cells from control animals were incubated in these "modified" media., their rate of proliferation almost approached that of the treated population. The results of the study suggest that mouse spleen cells responded to direct or indirect DPG treatment and that the mitogenic stimulus provided by DPG paralleled increased DNA synthesis during and after exposure to the compound.

Applicant's summary and conclusion

Conclusions:
The chronic exposure of C57Bl/J6 X DBA2 mice to DPG induced an enlargement of the spleen and a time-dependent increase of the DNA-synthesis in spleen cells.