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Ecotoxicological information

Long-term toxicity to fish

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Description of key information

A long-term study (34 days) was conducted on Pimephales promelas eggs (OECD 210 guidelines, semi-static method). NOEC for both hatching and larval survival rates were 1.3 mg/L

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
1.3 mg/L

Additional information

DPG (1,3-Diphenylguanidine) has been shown to be toxic to fish and algae and harmful to daphnia in several acute studies (fish : 96 h LC50 = 4.2-11 mg/l; algae : 72 h EC50 = 7.5 mg/l; 96 h EC50 = 1.7 mg/l; daphnid : 24 h EC50 = 73.6 mg/l; 48 h EC50 = 17mg/l). The PNEC can be determined using the NOECs from the algae (0.3 mg/l) and daphnid chronic 21 d (0.6 mg/l) studies (excluding the EbC50 results), by applying an uncertainty factor of 50. The resulting PNEC would be 6 µg/l. In order to refine this PNEC value for the aquatic compartment we performed a long-term test on fish (following OECD 210 guidelines).

In brief, Pimephales promelas eggs were exposed to graded concentrations of DPG for 34 days (0.041, 0.13, 0.41, 1.3 and 4.2 mg/L) under semi-static conditions. After 8 days, all larvae had hatched. Both hatching and larval survival rates were recorded daily. At the end of the test, all surviving larvae were weighted and measured. No significant effect were observed on fish length and weight. The NOEC for both hatching and larval survival was 1.3 mg/L. EC50 for larval survival was 1.8 mg/L.

From this result, the retained NOEC will be 0.3 mg/L (algae results).