Registration Dossier

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restriction
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1998

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
Deviations:
no
Principles of method if other than guideline:
NA
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
NA
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
NA
Radiolabelling:
no

Sampling and analysis

Details on sampling:
NA

Test solutions

Vehicle:
not specified
Details on preparation of test solutions, spiked fish food or sediment:
NA

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp (Cyprinus carpio)
- Source: Sugishima fish farm, Japan
- Length at study initiation (lenght definition, mean, range and SD): ca. 10 cm
- Weight at study initiation (mean and range, SD): ca. 30 g
- Method of breeding: Pelleted feed for carp

ACCLIMATION
- Acclimation period: 28 days
- Acclimation conditions (same as test or not): 25 °C +/- 1 °C

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk

Test conditions

Hardness:
NA
Test temperature:
25 °C +/- 2 °C
pH:
NA
Dissolved oxygen:
6 - 8 mg/L
TOC:
NA
Salinity:
NA
Details on test conditions:
- Test vessel:
- Material, size, headspace, fill volume: glass tanks of 100 liters in volume
- Type of flow-through (e.g. peristaltic or proportional diluter): Aquatron
- Renewal rate of test solution (frequency/flow rate): 200-800 ml/min
- No. of organisms per vessel: 15 - 20
Nominal and measured concentrations:
NA
Reference substance (positive control):
not specified
Details on estimation of bioconcentration:
NA

Results and discussion

Lipid content
Lipid content:
4 %
Time point:
start of exposure
Bioaccumulation factoropen allclose all
Type:
BCF
Value:
< 2.2
Basis:
other: Concentration of test substance in fish, no further data available
Remarks on result:
other: Conc.in environment / dose:50 microg/L
Type:
BCF
Value:
< 0.2
Basis:
other: Concentration of test substance in fish, no further data available
Remarks on result:
other: Conc.in environment / dose:500 microg/L
Details on kinetic parameters:
NA
Metabolites:
NA
Results with reference substance (positive control):
NA
Details on results:
NA
Reported statistics:
NA

Any other information on results incl. tables

NA

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The BCF for the test susbtsnce was measured to be very low (< 0.2 and < 2.2 at test concentrations of 500 and 50 µg/L). Thus, a bioaccumulation of the test susbstance in aquatic organisms is not expected.
Executive summary:

Materials and methods

A Bioaccumulation study to fish was conducted according to OECD Guideline 305 C. The species Cyprinus carpio were exposed to the test substance.over a period of 6 weeks at test concentrations of 50 and 500 µg/L in a flow through system

Results and discussion

The BCF for the test susbtsnce was measured to be very low (< 0.2 and < 2.2 at test concentrations of 500 and 50 µg/L). Thus, a bioaccumulation of the test substance to aquatic organisms is not expected.