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EC number: 208-534-8 | CAS number: 532-32-1
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Limited study summary, no guideline and no GLP.
- Justification for type of information:
- Please see Read Across Justification document in Section 13.2 of IUCLID
Cross-reference
- Reason / purpose for cross-reference:
- read-across source
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Study predates approved guidelines.
Five male and 5 female rats of the Spartan strain was used in this test. The group of 10 rats was placed in a sealed 59.1 liter glass chamber and exposed for 4 hours to a dynamic atmosphere containing the dust of 12200 mg/m3 test substance. - GLP compliance:
- no
- Test type:
- fixed concentration procedure
- Limit test:
- yes
Test material
- Reference substance name:
- Benzoic acid
- EC Number:
- 200-618-2
- EC Name:
- Benzoic acid
- Cas Number:
- 65-85-0
- Molecular formula:
- C7H6O2
- IUPAC Name:
- Benzoic acid
- Details on test material:
- Batch No.: 09-I2-28
Purity: not specified
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Spartan
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: from 226 to 244 grams
- Fasting period before study: not indicated
- Housing: Animals were housed by sex in groups of 5 in metal cages above the dropping.
- Diet (e.g. ad libitum): Purina Laboratory Chow
- Water (e.g. ad libitum): ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): controlled
- Humidity (%): controlled
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): no data
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- whole body
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Wright Dust Feeder
- Exposure chamber volume: 59.1 liter
- Method of holding animals in test chamber: closed chamber with seperated 4 units of 2 or 3 rats each
- Source and rate of air: Dried and filtered air was passed through the mechanism and directly into the exposure chamber.
- Method of conditioning air: Airflow was regulated by means of a flowmeter.
- System of generating particulates/aerosols: Wright Dust Feeder
- Method of particle size determination: not performed - Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 4 h
- Concentrations:
- approximately 12200 mg/m3 (The physical properties of the test material preclude the administration of the test compound at higher atmospheric concentrations.)
- No. of animals per sex per dose:
- 5 male and 5 female per dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 4 hr, 24 hr and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- none stated
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 12 200 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- There were no deaths following a single inhalation dose of the test substance at 12200 mg/m3 air.
- Clinical signs:
- other: Increased motor activity and slight erythema were observed during the 4 hour exposure period. After 4 hours exposure, 1 rat exhibited salivation. After 24 hours and after 14 days all animals appeared normal.
- Body weight:
- Normal body weight gain was observed.
- Gross pathology:
- No information provided.
- Other findings:
- No information provided.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The LC50 was considered to be >12,200 mg/m3 air. The test substance would not be considered a highly toxic material by the inhalation route of administration.
- Executive summary:
Five male and 5 female rats of the Spartan strain was used in this test. The group of 10 rats was placed in a sealed 59.1 liter glass chamber and exposed for 4 hours to a dynamic atmosphere containing the dust of 12200 mg/m3 test substance.
The LC50 was considered to be >12,200 mg/m3 air. The test substance would not be considered a highly toxic material by the inhalation route of administration.
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