Registration Dossier

Administrative data

Description of key information

Key value for chemical safety assessment

Skin sensitisation

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not sensitising)
Additional information:

The skin sensitisation test was carried out with female guinea pigs of Std:Hartley strain and female CBA/J mice (key study by Matsuoka et al. 2002). Guinea pigs were used in the maximisation test, whereas mice were used in the local lymph node assay. Strong irritation reaction was observed in all animals used in the maximisation test, which was comparable in test and control animals. From this it was concluded that chloroform was not sensitising to the skin of guinea pigs. In the local lymph node assay, chloroform tended to be higher abundant in the lymph nodes than the acetone/olive oil in the solvent control. The lymphoproliferative activity is used as an index of sensitisation in the LLNA, but since primary irritation also activates lymph cell proliferation through inflammatory cytokine effects, the reactions are said to be difficult to differentiate. Since Montelius et al. (1994) showed activation due to primary irritation in LLNA using methanol/chloroform as the vehicle, it is very likely that the reactions to chloroform seen in the Japanese LLNA study were due to primary irritation rather than sensitisation. In conclusion, chloroform should be considered as not sensitising to the skin.


Migrated from Short description of key information:
Only one study investigating the skin sensitisation potential of chloroform is available (Matsuoka et al. 2002). In this study, a maximisation test was performed in guinea-pigs and a local lymph node assay was conducted in mice. Chloroform was found not sensitising, but pronounced signs of irritation were observed.

Respiratory sensitisation

Endpoint conclusion
Additional information:

No signs of respiratory sensitisation have been reported.


Migrated from Short description of key information:
No signs of respiratory sensitisation have been reported.

Justification for classification or non-classification

Based on the available data in can be concluded that chloroform is not sensitising to the skin and no classification is proposed for chloroform.