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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: The study was not carried out according to generally accepted guidelines and the documentation is insufficient for an assessment of the quality of the study.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1976

Materials and methods

Principles of method if other than guideline:
Dermal application following Draize's method; interpretation of results and scoring following official scoring method for cosmetics
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
chloroform obtained from PROLABO with purity degree "R.P."

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
Female rabbits weighing between 2.4 and 3.0 kg

Test system

Type of coverage:
not specified
Preparation of test site:
not specified
Vehicle:
not specified
Controls:
no
Amount / concentration applied:
no data
Duration of treatment / exposure:
no data
Observation period:
no data
Number of animals:
six

Results and discussion

In vivo

Results
Irritation parameter:
primary dermal irritation index (PDII)
Basis:
mean
Time point:
other: no data
Score:
5.6
Reversibility:
no data
Irritant / corrosive response data:
Macroscopic observations included moderate to severe erythema and oedema with some superficial peripheral necrosis.

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information
Conclusions:
It can be concluded that chloroform is irritating to the rabbit's skin.
Executive summary:

The skin irritation potential of chloroform was tested in female New Zealand rabbits according to the principles of the Draize test. A single application of pure chloroform resulted in moderate to severe erythema and moderate to severe oedema. The primary dermal irritation index of chloroform was 5.6. Chloroform was classified as a severe skin irritant.