Registration Dossier
Registration Dossier
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EC number: 200-663-8 | CAS number: 67-66-3
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other:
- Version / remarks:
- Dermal application following Draize's method; interpretation of results and scoring following official scoring method for cosmetics.
- Principles of method if other than guideline:
- Dermal application following Draize's method; interpretation of results and scoring following official scoring method for cosmetics.
- GLP compliance:
- no
- Remarks:
- did not exist at the time
Test material
- Reference substance name:
- Chloroform
- EC Number:
- 200-663-8
- EC Name:
- Chloroform
- Cas Number:
- 67-66-3
- Molecular formula:
- CHCl3
- IUPAC Name:
- trichloromethane
- Details on test material:
- chloroform obtained from PROLABO with purity degree "R.P."
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- Female rabbits weighing between 2.4 and 3.0 kg
Test system
- Type of coverage:
- not specified
- Preparation of test site:
- not specified
- Vehicle:
- not specified
- Controls:
- no
- Amount / concentration applied:
- no data
- Duration of treatment / exposure:
- no data
- Observation period:
- no data
- Number of animals:
- six
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- primary dermal irritation index (PDII)
- Basis:
- mean
- Time point:
- other: no data
- Score:
- 5.6
- Reversibility:
- no data
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- other: no scores per time point available
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Remarks on result:
- other: no scores per time point available
- Irritant / corrosive response data:
- Macroscopic observations included moderate to severe erythema and oedema with some superficial peripheral necrosis.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- It can be concluded that chloroform is irritating to the rabbit's skin.
- Executive summary:
The skin irritation potential of chloroform was tested in female New Zealand rabbits according to the principles of the Draize test. A single application of pure chloroform resulted in moderate to severe erythema and moderate to severe oedema. The primary dermal irritation index of chloroform was 5.6. Chloroform was classified as a severe skin irritant.
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