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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Détermination de la concentration léthale50 des principaux hydrocarbures aliphatiques chlorés chez le rat
Author:
Bonnet P, Francin JP, Gradiski D, Raoul G, Zissu D
Year:
1980
Bibliographic source:
Archives des maladies professionnelles, de médecine du travail et de Sécurité Sociale 41(6-7), 317-321

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Male rats received whole-body exposure to chloroform vapours for 6 hours and were observed for 14 days subsequently.
GLP compliance:
no
Test type:
traditional method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroform
EC Number:
200-663-8
EC Name:
Chloroform
Cas Number:
67-66-3
Molecular formula:
CHCl3
IUPAC Name:
trichloromethane
Details on test material:
Chloroform of 99 % purity was obtained from Merck.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
Rats had a body weight between 130 and 200 g.

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Analytical verification of test atmosphere concentrations:
yes
Remarks:
gas chromatograph
Duration of exposure:
6 h
No. of animals per sex per dose:
12 male rats
Control animals:
no
Details on study design:
After exposure animals were observed for 14 days. All animals were subject to necropsy and gross pathology.
Statistics:
The LD50 value and the 95% confidence interval were determined based on the method of Bliss (1938).

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LC50
Effect level:
9.17 mg/L air
Based on:
test mat.
95% CL:
> 8.69 - < 9.7
Exp. duration:
6 h

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
Based on the results of this study, the 6 -h LC50 value determined was 9.17 mg/L. Upon recalculation, the 4 -h LC50 would be 10.5 mg/L.
Executive summary:

The acute inhalation toxicity of chloroform was tested in male Sprague-Dawley rats exposed to chloroform vapours during 6 hours followed by an observation period of 14 days; the 6 -h LC50 value determined was 9.17 mg/L. Upon recalculation, the 4 -h LC50 would be 10.5 mg/L.