Registration Dossier

Administrative data

Endpoint:
neurotoxicity: oral
Remarks:
other: neurobehavioural effects
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: No clear description of the method and of the criteria for the evaluation of behavioural effects is provided.

Data source

Reference
Reference Type:
publication
Title:
Behavioral toxicity of trihalomethane contaminants of drinking water in mice
Author:
Balster RL, Borzelleca JF
Year:
1982
Bibliographic source:
Environmental Health Perspectives 46, 127-136

Materials and methods

GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
ICR
Sex:
male
Details on test animals and environmental conditions:
male adult, 19-24 g of weight

Administration / exposure

Route of administration:
oral: drinking water
Vehicle:
unchanged (no vehicle)
Details on exposure:
Male ICR mice were tested: groups receiving 0, 3.1 or 31.1 mg/kg bw/day for 14 and 90 days were tested on swimming, cling, screen and hole-board; groups receiving 100 mg/kg bw/day for 30 days were tested on passive-avoidance learning, groups receiving 100 or 400 mg/kg bw/day for 60 days were tested on operant behavior and groups receiving 3, 10 or 30 mg/kg bw/day for 10 days were tested on taste aversion.
Duration of treatment / exposure:
14 or 90 days of exposure in experiment 1, 30 days in experiment 2, 60 days in experiment 3, 10 days in experiment 4
Frequency of treatment:
daily
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
0, 3.1, 31.1 in experiment 1
Basis:
other: doses in mg/kg/day received with water
Remarks:
Doses / Concentrations:
100
Basis:
other: doses in mg/kg/day received with water
Remarks:
Doses / Concentrations:
100, 400
Basis:
other: doses in mg/kg/day received with water
Remarks:
Doses / Concentrations:
3, 10, 30
Basis:
other: doses in mg/kg/day received with water

Results and discussion

Results of examinations

Details on results:
For most behavioural endpoints no significant difference was observed among treated and untreated animals. Changes in the operant behaviour and in taste aversion were recognised in the treated groups.

Applicant's summary and conclusion

Executive summary:

A test was carried out on the effects of oral chloroform exposure at 0, 3.1 or 31.1 mg/kg bw/day for 14 or 90 days on behavioural endpoints. For most behavioural endpoints no significant difference was observed among treated and untreated animals. Changes in the operant behaviour and in taste aversion were recognised in the treated groups.