Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 Sep - 15 Oct 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
yes
Remarks:
Day 5, two vessels: air flow fell to a minimum of 20 mL/min, which is slightly below the recommended 30 - 100 mL/min
Qualifier:
according to guideline
Guideline:
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
Deviations:
yes
Remarks:
Day 5, two vessels: air flow fell to a minimum of 20 mL/min, which is slightly below the recommended 30 - 100 mL/min
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Oakley sewage treatment works
- Laboratory culture: no
- Preparation of inoculum for exposure: homogenisation, filtration through Whatman GFC filter paper
- Concentration of sludge: 30 mg suspended solids/L
Duration of test (contact time):
29 d
Initial conc.:
10 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: Mineral salts medium
- Test temperature: 21.3 - 23.6 °C
- pH: 7.5-7.7
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes


TEST SYSTEM
- Culturing apparatus: 5 L glass culture bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: bubbling with CO2-free air (30-100 mL/min)
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: air outlet was connected to three Dreschel bottles in series, each containing 100 mL of 0.025 N of barium hydroxide


SAMPLING
- Sampling frequency: every second day
- Sampling method: 1st Dreschel bottle was disconnected and used for analysis; a fresh bottle was connected to the outlet at the end of the series


CONTROL AND BLANK SYSTEM
- Inoculum blank: bottles No. 1, 2
- Abiotic sterile control: no
- Toxicity control: bottle No. 6
- Other:
reference: bottle No. 3
test substance: bottles No. 4, 5


STATISTICAL METHODS: CO2/ThCO2

Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (CO2 evolution)
Value:
81
Sampling time:
29 d
Results with reference substance:
The reference substance sodium benzoate showed the expected biodegradation results (for details please see table below).

Sampling day

Degradation (% CO2/ThCO2)

Reference substance

Toxicity control

Test substance

Culture 1

Culture 2

Mean

2

19

23

1

1

1

4

54

54

28

27

28

6

65

63

40

39

40

8

72

-

49

48

49

10

76

-

57

55

56

12

79

-

62

59

61

14

80

-

67

64

66

19

82

-

77

73

75

24

82

-

82

78

80

28

83

-

82

79

81

29

83

-

82

79

81

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Butane-1,3-diol is readily biodegradable as demonstrated in the Modified Sturm Test performed according to OECD 301B.
Executive summary:

The ready biodegradability of 1,3 -butanediol was studied in a Modfied Sturm Test following OECD 301B. The test substance was degraded to 81% (CO2/ThCO2) after 29 days. The 10 -days window was met. The validity criteria were fulfilled. 1,3 -Butanediol is readily biodegradable according to OECD criteria.

Description of key information

Readily biodegradable as demonstrated in OECD TG 301 B (Barnes, 2000). 

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradability of 1,3 -butanediol was studied in a Modfied Sturm Test following OECD 301B. The test substance was degraded to 81% (CO2/ThCO2) after 29 days. The 10 -days window was met. The validity criteria were fulfilled. 1,3 -Butanediol is readily biodegradable according to OECD criteria (Barnes, 2000).