6-phenyl-1,3,5-triazine-2,4-diyldiamine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed according to OECD guideline study with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

OECD TG 471 & 472' Genetic Toxicology (Salmonella typhimurium and Escherichia coli)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

6-phenyl-1,3,5-triazine-2,4-diyldiamine

EC Number:

202-095-6

EC Name:

6-phenyl-1,3,5-triazine-2,4-diyldiamine

Cas Number:

91-76-9

Molecular formula:

C9H9N5

IUPAC Name:

6-phenyl-1,3,5-triazine-2,4-diamine

Test material form:

solid: particulate/powder

Details on test material:

solid

Specific details on test material used for the study:

purity: 98%

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Test concentrations with justification for top dose:

-S9: 0, 156, 313, 625, 1250, 2500, 5000 µg/plate;
+S9: 0, 156, 313, 625, 1250, 2500, 5000 µg/plate

Controls

Controls 1

Positive controls:

yes

Remarks:

-S9 mix

Positive control substance:

other: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide

Remarks:

TA100, TA98, WP2

Controls 2

Positive controls:

yes

Remarks:

-S9 mix

Positive control substance:

sodium azide

Remarks:

TA1535

Controls 3

Positive controls:

yes

Remarks:

-S9 mix

Positive control substance:

other: 9-aminoacridine hydrochloride

Remarks:

TA1537

Controls 4

Positive controls:

yes

Remarks:

+S9 mix

Positive control substance:

other: 2-aminoanthracene

Remarks:

all strains

Details on test system and experimental conditions:

Ames test, metabolic activation system: S9 from rat liver,induced with phenobarbital and 5,6-benzoflavone

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: Toxicity was not observed up to 5000 µg/plate in five strains with or without S9mix.

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: Toxicity was not observed up to 5000 µg/plate in five strains with or without S9mix.

Any other information on results incl. tables

GENOTOXIC EFFECTS: 
-with metabolic activation: 
   Salmonella typhimurium TA100, TA1535, TA98, TA537; negative
   Escherichia coli WP2 uvrA; negative
-without metabolic activation: 
   Salmonella typhimurium TA100,TA1535,TA98,TA537; negative
   Escherichia coli WP2 uvrA; negative
PRECIPITATION CONCENTRATION: 
   At the dose level more than 2500 µg/plate, visible precipitation was  shown at the end of exposure period.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

The presented study is reliable and adequte for the chemical safety assessment of Benzoguanamine.

Executive summary:

In a reverse gene mutation assay in bacteria, Salmonella typhimurium (TA100, TA98,TA1535, TA1537) and E. coli (WP2uvrA)  were exposed to this substance included DMSO at concentrations of 0, 156, 313, 625, 1250, 2500, 5000 µg/plate in the presence and absence of mammalian metabolic activation. 

 

This substance was tested up to limit concentration 5000µg/plate.  The positive controls did not induce the appropriate responses.  There was no evidence of induced mutant colonies over background.

 

This study satisfies the requirement for Test Guideline  OECD 471and 472 for in vitromutagenicity (bacterial reverse gene mutation) data.