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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin sensitisation

Currently viewing:

Administrative data

Endpoint:
skin sensitisation: in vivo (LLNA)
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was performed according to OECD guideline study with GLP compliance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
GLP compliance:
yes
Type of study:
mouse local lymph node assay (LLNA)

Test material

Constituent 1
Chemical structure
Reference substance name:
6-phenyl-1,3,5-triazine-2,4-diyldiamine
EC Number:
202-095-6
EC Name:
6-phenyl-1,3,5-triazine-2,4-diyldiamine
Cas Number:
91-76-9
Molecular formula:
C9H9N5
IUPAC Name:
6-phenyl-1,3,5-triazine-2,4-diamine

In vivo test system

Test animals

Species:
mouse
Strain:
CBA
Sex:
female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source:Harlan Laboratories, NL
- Age at study initiation: 8-12 weeks
- Weight at study initiation: 15-25 g
- Housing: group housing (Makrolon Type III)
- Diet (e.g. ad libitum): pelleted standard diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2 °C
- Humidity (%): 45-65 %
- Air changes (per hr): no information available
- Photoperiod (hrs dark / hrs light): 12 / 12

Study design: in vivo (LLNA)

Vehicle:
dimethyl sulphoxide
Concentration:
2.5%, 5%, 7.5%
No. of animals per dose:
4

Results and discussion

In vivo (LLNA)

Resultsopen allclose all
Parameter:
SI
Value:
1.11
Test group / Remarks:
Group 3 (5%)
Parameter:
SI
Value:
1.09
Test group / Remarks:
Group 4 (7.5%)
Parameter:
SI
Value:
1.44
Test group / Remarks:
2.5 % (Group 2)
Parameter:
other: disintegrations per minute (DPM)
Remarks on result:
other: Group 1 (control group): 225.6 Group 2 (2.5%): 325.5 Group 3 (5%): 251.0 Group 4 (7.5%): 245.5

Any other information on results incl. tables

Group 1 (control group): 1.00Group 2 (2.5%): 1.44Group 3 (5%): 1.11Group 4 (7.5%): 1.09

Applicant's summary and conclusion

Interpretation of results:
not sensitising
Remarks:
Migrated information
Conclusions:
The presented guideline study is reliable and adequate for the chemical safety assessment of 6-phenyl-1,3,5-triazine-2,4-diyldiamine. Based on the findings of the study the test substance is classified as not sensitising.