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EC number: 222-020-0 | CAS number: 3319-31-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 17.1, 30.9, 55.6, 100 and 180 mg/L
- Sampling method: No data
- Sample storage conditions before analysis: No data - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Tris(2-ethylhexyl)benzene-1,2,4-tricarboxylate was diluted to 1800mg/L (with solubilizer HCO-40 1000mg/L) with diluting water (Elendt M4) before use
- Eluate: dilution water
- Differential loading: No data
- Controls: yes - dilution water and test medium with dispersant
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): solubilizer HCO-40 1000mg/L
- Concentration of vehicle in test medium (stock solution and final test solution): 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Tris(2-ethylhexyl)benzene-1,2,4-tricarboxylate became cloudy in high concentration, but no precipitation was seen - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source:NIES Japan
- Age at study initiation (mean and range, SD): within 24h old
- Feeding during test: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48
- Post exposure observation period:
- No
- Hardness:
- 228 mg/l as CaCO3
- Test temperature:
- 19.9-20.2 °C (mean temperature 20°C).
- pH:
- 7.3-7.8
- Dissolved oxygen:
- 8.0-8.6 mg/l
- Salinity:
- N/A fresh water
- Nominal and measured concentrations:
- Nominal Concentrations: 0, 17.1, 30.9, 55.6, 100 and 180 mg/L
Mean measured concentrations: 0, 15.8, 28.9, 52.5, 97.3, 177.5 (See Results table below) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Material, size, headspace, fill volume: 100 ml
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): N/A static
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4(OECD guideline No.211 Annex 2)
- Intervals of water quality measurement: 48h
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16h light / 8h dark
- Light intensity: room light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca 1.8
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study
- Test concentrations: up to at least 1000 mg/l in immobilisation test
- Results used to determine the conditions for the definitive study:48h-EC50 for parent Daphnia (Acute immobilization test) was >1000mg/L. Dispersant control was also tested - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No abnormal responses observed during test period
- Other biological observations: No
- Mortality of control: No mortality observed during test period
- Other adverse effects control: No
- Abnormal responses: No abnormal responses observed during test period
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The Tris(2-ethylhexyl)benzene-1,2,4-tricarboxylate containing media became cloudy in high concentration, but no precipitation was seen
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50/LC50: 0.87 mg/L (48h) - Reported statistics and error estimates:
- Not applied for EC50 - No concentration related effects observed
Method of calculating mean measured concentrations: Geometric mean - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this test the EC50 (48h) > 180mg/L and NOEC (48h) > 180mg/L for Daphnia magna Tris(2-ethylhexyl)benzene-1,2,4-tricarboxylateor
- Executive summary:
Acute immobilisation in Daphnia has been investigated in a 48 hour test according to OECD test methods. Dissolution/dispersion agents were used to increase concentration of the substance in the test medium. The EC50 (48h) was determined to be >180 mg/L and the NOEC was 180 mg/L, the highest concentration investigated.
Reference
Measured Concentrations of test chemicals during a 48h exposure period
Nominal Concentration Measured concentration (mg/L) Percent of nominal concentration (mg/L)
0hr 48hr Mean 0hr 48hr
Control < 1.0 < 1.0 - - -
Disp.Cont. < 1.0 < 1.0 - - -
17.1 16.3 15.4 15.8 95.3 90.1
30.9 29.4 28.5 28.9 95.1 92.2
55.6 53.0 52.1 52.5 95.3 93.7
100.0 98.4 96.3 97.3 98.4 96.3
180.0 179.2 175.8 177.5 99.6 97.7
Mortality or immobility of Daphnia magna exposed to the test chemicals
Nominal concentration (mg/L) Cumulative number of dead or immobilized Daphnia
(Percent Mortality or Immobility)
24 hour 48 hour
Control 0(0) 0(0)
Dispersant Control 0(0) 1(5)
17.1 0(0) 1(5)
30.9 0(0) 0(0)
55.6 0(0) 0(0)
100.0 0(0) 0(0)
180.0 0(0) 0(0)
·
Description of key information
Short-term toxicity to Daphnia: EC50 - >180 mg/L; NOEC - 180 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- LC50
- Effect concentration:
- 180 mg/L
Additional information
Acute immobilisation in Daphnia has been investigated in a 48 hour test according to OECD test methods. Dissolution/dispersion agents were used to increase concentration of the substance in the test medium. The EC50 (48h) was determined to be >180 mg/L and the NOEC was 180 mg/L, the highest concentration investigated.
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