Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

3 hour EC50 > 1000 mg/L; 3 hour NOEC = 1000 mg/L, OECD 209, EU Method C.11, EPA OPPTS 850.6800,  Youngs (2010) 

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

A study was performed to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 209 "Activated Sludge, Respiration Inhibition Test", Method C.11 of Commission Regulation (EC) No. 440/2008 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.6800.

During the study activated sewage sludge was exposed to an aqueous dispersion of the test material at concentrations of 10, 32, 100, 320 and 1000 mg/L for a period of 3 hours at a temperature of 21 ± 1°C with the addition of a synthetic sewage as a respiratory substrate.

The rate of respiration was determined after 3 hours contact and compared to data for the control and a reference item, 3,5-dichlorophenol.

The effect of the test material on the respiration of activated sewage sludge gave a 3 -hourour EC50of greater than 1000 mg/L. The No Observed Effect Concentration (NOEC) after 3 hours exposure was1000mg/L.

It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/L.