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Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: insufficient for assessment: o-cresol only used in the re-challenge experiment

Data source

Reference
Title:
No information
Author:
Bruze M (1986). Sensitizing capacity of 2-methylolophenol 4-methylol phenol and 2,4,6-trimethylol phenol in the guinea pig. Contact Dermatitis 14, 32-38

Materials and methods

Principles of method if other than guideline:
GPMT was first performed with 2-methylolphenol, then re-challenge was performed with 13.1 w/w% o-cresol in ethanol.
GLP compliance:
not specified
Type of study:
guinea pig maximisation test

Test material

Constituent 1
Chemical structure
Reference substance name:
o-cresol
EC Number:
202-423-8
EC Name:
o-cresol
Cas Number:
95-48-7
Molecular formula:
C7H8O
IUPAC Name:
o-cresol
Details on test material:
o-cresol, no data on purity

In vivo test system

Test animals

Species:
guinea pig
Strain:
Dunkin-Hartley
Sex:
female
Details on test animals and environmental conditions:
no data

Study design: in vivo (non-LLNA)

Inductionopen allclose all
Route:
intradermal
Vehicle:
other: ethanol
Concentration / amount:
intradermal : 5.0 % w/v: SLS: 10 %% w/v; Topical 25 %w/v; Challenge (I)15 % (II) o-cresol 13.1 %w/v
Challengeopen allclose all
Route:
epicutaneous, occlusive
Vehicle:
other: ethanol
Concentration / amount:
intradermal : 5.0 % w/v: SLS: 10 %% w/v; Topical 25 %w/v; Challenge (I)15 % (II) o-cresol 13.1 %w/v
No. of animals per dose:
no data
Details on study design:
According to Magnusson and Kligman, J. Invest Dermatol. (1969), 52, 268-276.
Challenge controls:
yes
Positive control substance(s):
not specified

Results and discussion

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Executive summary:

Guinea pigs sensitized with 2-methylol phenol showed skin effects also with o-cresol (13.1%).
Control group animals also showed skin effects, therefore the used test concentration was probably irritating.