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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable publication

Data source

Reference
Reference Type:
publication
Title:
Ethylene Glycol Ethers as Hemopoietic Toxins in vitro Studies of Acute Exposure
Author:
Ruchand S, Boiron O, Cicolella A, Lanotte M
Year:
1992
Bibliographic source:
Leukemia 6(4) 328-334

Materials and methods

Principles of method if other than guideline:
Method: other: In vitro toxicity of 2-ethoxyethanol analyzed on several hemopoietic cell lines.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of the test substance as stated in the publication: ethylene glycol monoethyl ether (EGEE);
purchased from: Aldrich Chemical Co. (Milwaukee, WI, USA)

Results and discussion

Any other information on results incl. tables

2 -ethoxyethanol was tested for 48 h at various concentrations between 0.1 M and 0.1 µM, using serial half-dilutions. Dose-response curves showed the common fearture of a very steep slope on a semi-log plot, indicating that cell viability went from normal to nil by increasing concentration. This steep slope and the sigmoid shape of the curve suggested that the substance hindered some cellular mechanisms essential for cell survival.

Hematopoietic toxicity of 2 -ethoxyethanol:

               IC50 (mM) with cell lines
Compound   NB4  IPC-81  WEHI-3b  DA1  NFS-60
2 -ethoxyethanol   1.6  1.5  9.0  11.3  2.6

IC50 for 48 h incubation corresponded to the concentration (mM) for which the cell viability in treated culture were 50 % of the viability in untreated control cultures.

IC50 for 48 h incubation corresponded to the concentration (mM) for which the cell viability in treated culture were 50 % of the viability in untreated control cultures.

Applicant's summary and conclusion

Conclusions:
The test substance 2-ethoxyethanol was found to be a hemopoietic toxin and acted directly on the hemopoietic cells, either growth-factor-dependent or leukemic from rat, mouse and human species.