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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
also cited in OECD SIDS
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.4 +/- 0.3 °C
pH:
7.9 - 8.1
Dissolved oxygen:
7.8 - 8.1 mg/L
Details on test conditions:
Intensity of irradiation: Photoperiod: 16 hours light/8 hours darkness.
Total amount of test solution was replaced at 24 hours.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.3 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
not specified
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
1.69 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.948 mg/L
Nominal / measured:
meas. (geom. mean)
Details on results:
The concentrations of test material measured at preparation and after 24 hours were greater than +/- 20 % of the set-up values, therefore, the measured values (geometric average) were used.
Validity criteria fulfilled:
not applicable
Conclusions:
The toxicity of invertebrates (Daphnia magna) of TBBS was determined at an EC50 of 1.3 mg/L after a period of 48 hours. The toxicity was observed to be greater than the limit of water solubility of TBBS.
Executive summary:

The toxicity of invertebrates (Daphnia magna) of TBBS was determined according at the OECD guideline 202 "Daphnia, Acute Immobilisation Test" and showed an EC50 of 1.3 mg/L after a period of 48 hours. The toxicity was observed to be greater than the limit of water solubility of TBBS.

Description of key information

The toxicity of invertebrates (Daphnia magna) of TBBS was determined at an EC50 of 1.3 mg/L after a period of 48 hours. The toxicity was observed to be greater than the limit of water solubility of TBBS.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1.3 mg/L

Additional information

Due to fast hydrolysis of TBBS the observed effect is most probably a combined effect from both, TBBS and MBT. Therefore, the value determined for TBBS was chosen as key study. However the effective concentration on Daphnia magna reported in the study for MBT is less sensitive than the one reported for TBBS on algae, and hence the value for MBT was not used in further risk assessment for the PNEC estimation.