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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
also cited in OECD SIDS
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
yes
Test organisms (species):
Oryzias latipes
Details on test organisms:
Size: 1.59-2.30 cm
Weight: 0.059-0.217 g
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
24°C +/- 1°C
pH:
7.2 - 7.9
Dissolved oxygen:
5.0 - 8.3 mg/l
Details on test conditions:
Concentrations: Control, vehicle control, 0.25, 0.5, 1.0, 2.0, 4.0 mg/L. Total amount of test water was replaced every 24 hours. Test was performed in 5 L test vessel per concentration with 10 fish per vessel. Irratiation was room light, 16 hours light and 8 hours dark.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.518 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.38 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
3.99 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The measured concentrations compared to the set-up concentrations exceeded +/-20 %, hence values were based on the geometric mean of the measured concentrations.

Abnormal swimming was observed at test concentrations over 0.518 mg/L (set-up concentration 1.00 mg/L). No abnormal symptoms were seen in the control groups during the exposure period.

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity to fish ( Orizias latipes) of TBBS was determined after 96 hours at a LC50 of 1.38 mg/L under semi-static conditions.
Executive summary:

Following the OECD Guideline 203 a 96h-LC50 of 1.38 mg/L was observed on Oryzias latipes.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
also cited in OECD SIDS
Qualifier:
according to
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
concentrations measured at day 0, 7 and 14
Vehicle:
yes
Test organisms (species):
Oryzias latipes
Details on test organisms:
Size: 1.72-2.48 cm
Weight: 0.072-0.222 g
Loading: 10 fish per dose level
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
14 d
Test temperature:
24°C +/- 2°C
pH:
7.4 - 7.9
Dissolved oxygen:
5.5 - 8.2 mg/L
Nominal and measured concentrations:
concentrations: 0.049, 0.150, 0.440, and 1.30 mg/L
Details on test conditions:
Concentrations: Control, vehicle control, 0.049, 0.150, 0.440, and 1.30 mg/L.
Dosing rate: Continuous
Renewal of test solution: 6 times/day.
Test was performed in 5 L test vessel per concentration with 10 fish per vessel.
Irratiation was room light, 16 hours light and 8 hours dark.
Reference substance (positive control):
no
Duration:
14 d
Dose descriptor:
LC0
Effect conc.:
0.15 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
1.02 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LC100
Effect conc.:
1.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LOEC
Effect conc.:
0.44 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The ratios of the concentrations measured to the set-up concentrations were within +/- 20 % in all the test doses. All test concentrations were therefore based on nominal test substance concentrations.
Validity criteria fulfilled:
not applicable
Conclusions:
The prolonged toxicity to fish (Oryzias Latipes) was determined at a LC50 of 1.02 mg/L after 14 days. A LOEL after 14 days of 0.44 mg/L was calculated.
Executive summary:

Following the OECD Guideline 204 a 14d-LC50 of 1.02 mg/L was observed on Oryzias latipes.

Description of key information

Following the OECD Guideline 203 a 96h-LC50 of 1.38 mg/L was observed on Oryzias latipes (National Institute of Technology and Evaluation, 1996).
According to the OECD Guideline 204 a 14d-LC50 of 1.02 mg/L was observed on Oryzias latipes (National Institute of Technology and Evaluation, 1996).

Key value for chemical safety assessment

LC50 for freshwater fish:
1.38 mg/L

Additional information

A hydrolysis half-life time of TBBS of 7.76 – 9.53 h was observed in deionised and environmental water buffered at pH 7.0. Benzothiazole-2-thiole (MBT) was determined as hydrolysis product. However, the effect concentrations of TBBS to Oryzias latipes are chosen as key studies, since the observed effects are most probably a combined effect from both TBBS and MBT. The effect concentration on fish reported in the study for MBT (0.73 mg/L) is less sensitive than the reported value for TBBS on algae (0.071 mg/L), and hence the value for MBT is not used in further risk assessment for the PNEC estimation.