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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: No analytic was performed. Nominal test concentrations exceeded the solubility of the test item.
Principles of method if other than guideline:
Fish test, method: DIN 38412 part 15
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- Concentration of vehicle/ solvent: no vehicle or solvent
- Other procedures: no stock solution, direct weighing of test substance  for test solutions
Test organisms (species):
Leuciscus idus
Details on test organisms:
- Common name: Leuciscus idus
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none
Nominal and measured concentrations:
Concentrations: 500; 600; 800 mg/L (nominal)
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
>= 800 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 800 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Test parameter: mortality
- Behavioural abnormalities: no
Validity criteria fulfilled:
not specified
Conclusions:
The LC50 (48h) based on nominal concentrations was determined as > 800 mg/L indicating that the test substance is not harmful to fish.
Executive summary:

An acute toxicity study with Leucisus idus was performed according to the DIN 38412 part 15 method, under static conditions (48 h exposure duration). The fish were exposed to three concentrations: 500, 600, 800 mg dodecanedioic acid/L. No toxic reference or control treatment group was included in the test. No analytical monitoring was included. There were no mortalities, toxic effects or abnormal responses. Thus the LC50 (48 h) was >800 mg dodecanedioic acid /L.

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Abstract. No analytic was performed. Nominal test concentrations exceeded the solubility of the test item.
Principles of method if other than guideline:
Fish test, method: DIN 38412 part 15
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- Concentration of vehicle/ solvent: no vehicle or solvent
- Other procedures: Use of the disodium salt due to its higher solubility  in comparison with the diacid
Test organisms (species):
Leuciscus idus
Details on test organisms:
- Common name: Leuciscus idus
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none
Nominal and measured concentrations:
Concentrations: 200, 500, 1000 mg/l disodium salt
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
>= 840 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 840 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Test parameter: mortality
- Behavioural abnormalities: no
Sublethal observations / clinical signs:

Effect concentration vs. test substance solubility: 1000 mg disodium salt/L (molecular weight 274.27 g/mol) corresponds to 840 mg diacid/L (230.31 g/mol)

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 (48h) based on nominal concentrations was determined as > 840 mg/L indicating that the test substance is not harmful to fish.
Executive summary:

An acute toxicity study with Leuciscus idus was performed according to the DIN 38412 part 15 method, under static conditions (48 h exposure duration). The fish were exposed to three concentrations: 200, 500, 1000 mg disodium salt/L. Effect concentration vs. test substance solubility: 1000 mg disodium salt/L (molecular weight 274.27 g/mol) corresponds to 840 mg diacid/L (230.31 g/mol).

No toxic reference or control treatment group was included in the test. No analytical monitoring was included. There were no mortalities, toxic effects or abnormal responses. Thus the LC50 (48 h) was >840 mg dodecanedioic acid /L.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: No analytic was performed. Nominal test concentrations exceeded the solubility of the test item.
Principles of method if other than guideline:
Fish test DIN 38412 L 15
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Leuciscus idus
Details on test organisms:
- Common name: Golden orfes
- Length at study initiation (length definition, mean, range and SD): about 6 cm
- Feeding during test: no
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
none
Hardness:
about 16 ºdH
Test temperature:
20 ºC +/- 1 ºC
Nominal and measured concentrations:
Nominal: 1000 mg/L
Details on test conditions:
- Test vessel: fish basin containing 10 L test water
- Photoperiod: about 16 hours illumination per day
- Number of replicates: 1
- Number of fish per replicate: 10
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Test parameter: mortality
- Behavioural abnormalities: no
Validity criteria fulfilled:
not specified
Conclusions:
The LC50 (48h) based on nominal concentration was determined as > 1000 mg/L indicating that the test substance is not harmful to fish.
Executive summary:

An acute toxicity study with golden orfes was performed according to the DIN 38412 L 15 method (corresponding to the OECD-Guideline 203), under static conditions (48 h incubation period). 10 fish in one replicate were exposed to 1000 mg dodecanedioic acid /L nominal concentration. No toxic reference or control treatment group was included in the test. No analytical monitoring was included.

There were no mortalities, toxic effects or abnormal responses. Thus the LC50 (48 h) was determined as >1000 mg dodecanedioic acid /L.

Description of key information

A 48-h acute fish test was conducted by Scheubel J.R. and Scholz N. (1987), using DIN 38412 part 15 method, with Leuciscus idus (static). Concentrations: 200; 500; 1000 mg/L disodium salt were tested. A second acute fish test (48 h) conducted by Scheubel J.R. (1985) studied the toxicity of dodecanedioic acid on Leucisus idus (determined in a static test according to the DIN 38412 part 15 method). Concentrations: 500; 600; 800 mg/L were tested. 
An acute toxicity study (48h) was conducted by Wind T. and Mühlberg B. (1982) according to the DIN 38412 L 15 method, with Leuciscus idus (10 fish in 1000 mg dodecanedioic acid /L nominal concentration). It was performed under static conditions with one replicate per concentration level. No effects were observed on Leuciscus idus in any of the three tests. The lowest LC50 (48 hours) was >800 mg/L.

Key value for chemical safety assessment

Additional information

The results are summarised in the following table:

  Species Endpoint (exposure duration) Exposure concentration  Reference
 Leuciscus idus  LC50 (48 h)  > 840 mg/L  Scheubel J.R., Scholz N. (1987)
 Leuciscus idus   LC50 (48 h)  > 800 mg/L  Scheubel J.R. (1985)
 Leuciscus idus   LC50 (48 h)  > 1000 mg/L  Wind T., Mühlberg B. (1982)

The available studies indicate that dodecanedioic acid concentrations up to 1000 mg/L are not toxic to fish. However, taking into considaration that no analytical monitoring was conducted and that the water solubilty is between 30 mg/L and 40 mg/L the endpoint that should be considered for risk assessment purposes should be much lower than presented above .