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EC number: 253-733-5 | CAS number: 37971-36-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979-08
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Only four instead of at least five strains of bacteria were used.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 26 May 1983
- Deviations:
- yes
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- Directive 84/449/EEC
- Deviations:
- yes
- Principles of method if other than guideline:
- Only four instead of at least five strains of bacteria were used. It has to be mentioned that the test was performed in 1979, the current guideline was adopted in 1997.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-phosphonobutane-1,2,4-tricarboxylic acid
- EC Number:
- 253-733-5
- EC Name:
- 2-phosphonobutane-1,2,4-tricarboxylic acid
- Cas Number:
- 37971-36-1
- Molecular formula:
- C7H11O9P
- IUPAC Name:
- 2-phosphonobutane-1,2,4-tricarboxylic acid
- Details on test material:
- - technical pure product (category AC)
- 45-50% (w/w) 2-phosphonobutane-1,2,4-tricarboxylic acid in water
- Pt. 10 E 2806
Constituent 1
Method
- Target gene:
- The Salmonella typhimurium strains are constructed to differentiate between base pair (TA 1535, TA 100) and frameshift (TA 1537, TA 98) mutations. All stains show increased sensitivity due to "deep rough" (one polysaccharide chain is missing, hence, also large molecules can penetrate into the bacteria cells ) and UV-repair-deficiency.
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- NA
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- nominal concentrations: 20; 100; 500; 2500; 12,500 µg /plate
- Vehicle / solvent:
- - DMSO (BAHIBIT AM; Trypaflavin)
- demineralised water (Endoxan)
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- 0 µg BAHIBIT AM /plate / demineralised water
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- Substance name: Endoxan; cytostatic agent; the active agent cyclophosphamid is a well-known pro-mutagen; 217. 5 µg equivalent to 150 µg cyclophosphamid; only for strains 1535 and TA 100
- Negative solvent / vehicle controls:
- yes
- Remarks:
- demineralised water
- Positive controls:
- yes
- Positive control substance:
- other: Trypaflavin
- Remarks:
- Active substance of Panflavin and Rivanols; pro-mutagen for frameshift mutations; 200 µg; only for strains 1537 and TA 98
- Details on test system and experimental conditions:
- Incubation for 48h at 37°C.
4 plates / substance / strain
Negative controls:
4 plates / DMSO / strain
4 plates / demineralised water / stain
Additionally, the total germ account was determined for 2 plates/group. - Evaluation criteria:
- A positive result requires a reproducible dose-related duplication of mutants compared with the negative controls for at least one strain.
The colony numbers for negative controls, positive controls and the test plates were determined, the mean values were calculated. - Statistics:
- Mean values
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: only weak bacteriotoxic effects for concentrations >500 µg/ plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- NA
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No remarks.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
BAYHIBIT AM containing 45-50% (w/w) 2-phosphonobutane-1,2,4-tricarboxylic acid in water was found to be non-mutagenic under test conditions. - Executive summary:
The Ames test was performed with BAYHIBIT AM containing 45-50% (w/w) 2-phosphonobutane-1,2,4-tricarboxylic acid in water, at nominal concentrations of 16; 80; 400; 2500 and 12,500 µg /plate. Strain-specific bacteriotoxicity was observed at doses >500 µg/ plate and higher. The reported data of this mutagenicity assay shows, that under the experimental conditions reported, the test item did not induce gene mutations by frameshift or base-pair substitution strains used. Hence, BAYHIBIT AM containing 45-50% (w/w) 2-phosphonobutane-1,2,4-tricarboxylic acid is considered non-mutagenic in this bacterial reverse mutation assay.
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