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Data on the effect of C.I. Pigment Yellow 34 and C.I. Pigment Red 104 on terrestrial organisms are not available. Generally the substances are very stable in the environment. However, a low proportion of the substance may dissociate and release chromate and lead ions to the environment. The bioavailability of these ions depends on factors like pH and the presence of organic matter or mineral particles c.f. chapter 5.4. Thus, the availability of lead and chromate ions released by the substance is probably very limited.

Results on the toxicity of lead and chromate on terrestrial organisms indicate that both ions have detrimental effects on terrestrial flora and fauna. Both moieties of the pigments are taken up by earthworms. EC50 values that are only available for chromium predict a mortality rate of 50 % at a concentration of 20 mg Cr/l.