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EC number: 203-794-9 | CAS number: 110-71-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August-September 1981
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well performed guideline study (GLP/OECD)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,2-dimethoxyethane
- EC Number:
- 203-794-9
- EC Name:
- 1,2-dimethoxyethane
- Cas Number:
- 110-71-4
- Molecular formula:
- C4H10O2
- IUPAC Name:
- 1,2-dimethoxyethane
- Details on test material:
- No data
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0, 0.004, 0.02, 0.1, 0.5, 2.5 and 10 µL
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 9-Aminoacridine, 2-Aminoanthracene, Methylhydrazone Derivative, Streptozotozine, ENNG
- Details on test system and experimental conditions:
- The tester strains Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538 and E. coli WP2 uvrA were maintained in liquid nitrogen and recultivated for the test in fresh nutrient broth.
To 2 mL of molten (45°C) top agar in a sterile test-tube were added 0.1 mL of the tester strain culture, graded quantities of the test compound in 0.1 mL solution and, for the S9 series, 0.5 mL S9 mix. The contents of the test-tube were rapidly mixed and poured onto the surface of previously prepared minimal agar plates with Vogel-Bonner E mixture.
The plates (4 per tester strain and substance concentration) were incubated upside down at 37°C for 2 days, after which the number of revertant colonies appearing was counted.
Control plates without admixture of test compound were prepared in the same way to determine the spotaneous mutation rate. - Evaluation criteria:
- No data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Ames Test Ethylene glycol dimethyl ether
Table 1:Summary of mean number of revertants (mean of at 4 plates) in Salmonella typhimurium with and without metabolic activation (negative and positive controls) and Escherichia coli WP2 uvrA
Concentration |
TA 98 |
TA 100 |
TA1535 |
TA 1537 |
||||||||
[µL/plate] |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
||||
0 |
30 |
27.8 |
104.8 |
114.3 |
11.8 |
9.3 |
8.5 |
13.5 |
||||
10 |
37.5 |
36.8 |
130.8 |
130.5 |
15.5 |
13.3 |
11.5 |
14.3 |
||||
2.5 |
36.3 |
26.3 |
122 |
121 |
13.8 |
12.3 |
9 |
12.8 |
||||
0.5 |
31.5 |
35 |
114.3 |
123.3 |
15 |
10.5 |
9.5 |
11.3 |
||||
0.1 |
35.5 |
35.5 |
111.8 |
126 |
13.5 |
9.3 |
11.3 |
14 |
||||
0.02 0.004 |
35 29.5 |
29.8 25.8 |
112.8 110.8 |
117.3 121.5 |
16 12 |
10.8 10.3 |
9.3 11.5 |
15 13 |
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
9-Aminoacridine |
|
|
|
|
|
|
3750 |
|
||||
2-Aminoanthracene |
31 |
1028 |
102.5 |
959.5 |
11.5 |
191 |
12.5 |
141.5 |
||||
Methylhydrazone Deriv. |
3550 |
|
3100 |
|
|
|
|
|
||||
9-Aminoacridine Streptozotozine ENNG |
|
|
|
|
>5000 |
|
|
|
||||
|
||||||||||||
Concentration |
TA 1538 |
WP2 uvrA |
|
|
||||||||
[µL/plate] |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
|
|
|
|
||||
0 |
19 |
26.3 |
18.5 |
36.3 |
|
|
|
|
||||
10 |
19.3 |
24.5 |
24.3 |
38 |
|
|
|
|
||||
2.5 |
16.5 |
25.5 |
24.5 |
41 |
|
|
|
|
||||
0.5 |
15.8 |
26.3 |
19 |
34.3 |
|
|
|
|
||||
0.1 |
16 |
18 |
21 |
33.8 |
|
|
|
|
||||
0.02 0.004 |
15.5 21.3 |
24.8 25.5 |
25.5 20.8 |
37.3 38 |
|
|
|
|
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
9-Aminoacridine |
|
|
|
|
|
|
|
|
||||
2-Aminoanthracene |
14.5 |
1304 |
34.5 |
3850 |
|
|
|
|
||||
Methylhydrazone Deriv. |
>5000 |
|
|
|
|
|
|
|
||||
9-Aminoacridine Streptozotozine ENNG |
|
|
582 |
|
|
|
|
|
||||
Applicant's summary and conclusion
- Conclusions:
- negative with and without metabolic activation
Ethylene glycol dimethyl ether did not cause any increase in the number of revertant colonies with any of the tester strains in absence or presence of S9 mix. - Executive summary:
Ethylene glycol dimethyl ether was tested for mutagenicity with the strains TA1538, TA1537, TA1535, TA100 and TA98 of Salmonella thyphimurium and Escherichia coli WP2 uvrA.
The mutagenicity study was conducted in the absence and presence of a metabolising system derived from rat liver homogenate. A concentration of 0.004 to 10 µL Ethylenglycoldimethylether/plate was used.
Control plates without mutagen but with Dimethylsulfoxide did not show an increase in the number of revertant colonies compared to the spontaneous negative control. All positive control compounds gave the expected increase in the number of revertant colonies.
Ethylene glycol dimethyl ether did not show an increase in the number of revertants in any of the bacterial strains in the presence as well as in the absence of metabolic activation.
Therefore, it can be stated that Ethylene glycol dimethyl ether is not mutagenic in these bacterial test systems either with or without metabolic activation.
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