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EC number: 290-754-9 | CAS number: 90218-76-1
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-06-18 to 1997-07-09
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 202 Part 2
- Deviations:
- yes
- Remarks:
- In the development of new EC-methods the test conditions deviate from the OECD-Guideline. The evaluation is adopted to the EC-guideline. No influence on the results is expected
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- In the development of new EC-methods the test conditions deviate from the OECD-Guideline. The evaluation is adopted to the EC-guideline. No influence on the results is expected
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- For determination of stability of the test substance solution, a solution in deionized water was made and analyzed after 0 and 72 hours.
The stock solution for the test was deep-frozen at -18°C and reanalyzed every week during the test. - Vehicle:
- no
- Details on test solutions:
- As the test substance is poorly soluble in water, a 1 g/L mixture in M4 medium without NaHCO3 and FeSO4 containing Na2EDTA was prepared. This mixture was stirred for 18 hours and filtrated afterwards. The filtrate showed an initial content of 4.1 mg/L DOC. The filtrate was used as Stock solution.
For changes of test solutions every second or third day during the test the stock solution was deep-frozen at -18°C. One day before application the required amount of the frozen stock solution was thawed and after sampling for analytical purpose mixed with NaHCO3 and FeSO4 containing Na2EDTA. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, clone 5
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in synthetic fresh water (M4 medium, Elendt, 1990). A reddish dark-brown coloration of the animals indicated that they were healthy.
- Feeding during test: daily with Scenedesmus subspicatus (days 0 to 6: 0.5x10E7 cells/daphnid; days 7 - 21: 1x10E7 cells/daphnid)
ACCLIMATION
- Acclimation period: Females were seperated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: not mentioned
- Feeding frequency: not mentioned - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Post exposure observation period:
- none
- Hardness:
- not mentioned
- Test temperature:
- 20 ± 1 °C
- pH:
- 7,6 - 8,2 over all test vessels. In one case a pH deviation of > 0.3 Units was found between the freshly prepared solution and the 2-3 day old solutions. As the reproduction rate was not impaired by this deviation, it was considered as not affecting the validity of this test.
- Dissolved oxygen:
- 92% to 107% of saturation
- Salinity:
- according to guideline (M4 medium, Elendt)
- Nominal and measured concentrations:
- Nominal: WSF
Measured: 4.5 mg/L (mean of measured concentrations,concentration of test solutions remained stable during 72 hours: deviations < 20%) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Glass beakers, 100 mL filled with 60 mL test solution
- Aeration: not mentioned
- Renewal rate of test solution (frequency/flow rate): 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 1 organism/60 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water according to Elendt M4-Medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Day 0 (fresh samples), 2, 9, 12, 14, 16
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light, 8h dark
- Light intensity: light: 600 lux, dark: 100 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality and offspring on day 2, 5, 7, 9, 12, 14, 16, 19, 21
VEHICLE CONTROL PERFORMED: no - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 4.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- reproduction
- Reported statistics and error estimates:
- As the reproduction rate with test substance was higher than in the control samples, no statistic has been performed.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest tested concentration at which no negative effects were observed on the reproduction rate or survival of the parent animals is:
NOEC >= 4.5 mg/L (highest concentration under test conditions)
The lowest tested concentration at which the first significant effects were observed on the reproduction rate or survival of the parent animals is:
LOEC > 4.5 mg/L (highest concentration under test conditions)
The concentrations were based on the product. - Executive summary:
The effect of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters on the immobilisation of Daphnia magna had been studied in accordance with OECD Guideline for Testing of Chemicals No. 202 part II.
Daphnia magna was exposed to 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters in a semistatic reproduction test over 21 days. As the test substance is poorly soluble in water, a 1 g/L mixture with synthetic freshwater was prepared. This mixture was stirred for 18 hours and filtrated afterwards. For changes of test solutions during the test the stock solution was deep-frozen at -18°C. The analytical determination of the defrosted stock solutions used for the test showed that they were stable within the 3 days of exposure (deviation > 20%), therefore the mean of the measured values (4.5 mg/L) were used for evaluation.
The highest tested concentration at which no negative effects were observed on the reproduction rate or survival of parental animals is: NOEC >= 4.5 mg/L (highest test concentration under test conditions).
The lowest tested concentration at which the first significant effects were observed on the reproduction rate or survival of the parent animals is: LOEC > 4.5 mg/L (highest concentration under test conditions).
The concentrations were based on the product.
Reference
Table #1: Number of living parent animals
| Conc. [mg/L] | Parallel | 2d | 5d | 7d | 9d | 12d | 14d | 16d | 19d | 21d | Day 21 |
control |
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Total parent animals 9 |
| 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 4 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| 6 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 7 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 8 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 9 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 10 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
4.5 |
1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Total parent animals 9 |
| 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 3 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 4 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 6 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 7 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 8 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 9 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
| 10 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
Table #2: Number of living offspring
| conc. [mg/L] | parallel | 21 day total | 21 day mean |
| control | 1 | 89 | mean value offspring per parent animal 74 |
| 2 | 61 | ||
| 3 | 63 | ||
| 4 | 88 | ||
| 5 | - | ||
| 6 | 84 | ||
| 7 | 69 | ||
| 8 | 76 | ||
| 9 | 70 | ||
| 10 | 66 | ||
| 4.5 | 1 | 80 | mean value offspring per parent animal 78 |
| 2 | 69 | ||
| 3 | 85 | ||
| 4 | 79 | ||
| 5 | 75 | ||
| 6 | 79 | ||
| 7 | 78 | ||
| 8 | 79 | ||
| 9 | 80 | ||
| 10 | - |
Description of key information
The highest tested concentration at which no negative effects were observed on the reproduction rate or survival of the parent animals is:
NOEC = 4.5 mg/L (highest concentration under test conditions)
The lowest tested concentration at which the first significant effects were observed on the reproduction rate or survival of the parent animals is:
LOEC > 4.5 mg/L (highest concentration under test conditions)
The concentrations were based on the product.
The test substance was not toxic under test conditions in the range of water solubility.
Key value for chemical safety assessment
Additional information
The highest tested concentration at which no negative effects were observed on the reproduction rate or survival of the parent animals is: NOEC >= 4.5 mg/L (highest concentration under test conditions) The lowest tested concentration at which the first significant effects were observed on the reproduction rate or survival of the parent animals is: LOEC > 4.5 mg/L (highest concentration under test conditions) The concentrations were based on the product.
The test substance was not toxic under test conditions in the range of water solubility.
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