3-aminopropyldimethylamine

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25. Oct 1956 - 07. Nov 1956

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1961

Report date:

1961

Materials and methods

Principles of method if other than guideline:

BASF-Test:
50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 3h on the day of treatment and up to 14 days afterwards. Findings were recorded every 2-3 days. The eyes were not washed out after 24 hours as specified in OECD Guideline 405. The report describes findings after 1 and 24 hours and at the end of the observation period.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-aminopropyldimethylamine

EC Number:

203-680-9

EC Name:

3-aminopropyldimethylamine

Cas Number:

109-55-7

Molecular formula:

C5H14N2

IUPAC Name:

N,N-dimethylpropane-1,3-diamine

Test material form:

liquid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.57 kg (mean)

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The adjacent eye served as saline control.

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1 drop

Duration of treatment / exposure:

14 days

Observation period (in vivo):

14 days

Number of animals or in vitro replicates:

2

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Remarks:

15 min

Basis:

mean

Remarks:

animal 1-2

Time point:

24 h

Score:

4

Max. score:

4

Reversibility:

not reversible

Remarks on result:

probability of severe irritation

Remarks:

Burn after 8 days of destruction of the eye

Results 2

Irritation parameter:

other: conjunctiva findings

Time point:

other: after 10 minutes up to 6 days

Reversibility:

not reversible

Remarks:

in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration

Remarks on result:

other: brown-reddish discoloration of the conjunctiva with bloody secretion

Results 3

Irritation parameter:

other: chemosis

Time point:

other: after 10 minutes up to 6 days

Reversibility:

not reversible

Remarks:

in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration

Remarks on result:

other: slight chemosis

Results 4

Irritation parameter:

other: corneal findings

Time point:

other: after 10 minutes up to 6 days

Reversibility:

not reversible

Remarks:

in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration

Remarks on result:

other: Opacity

Results 5

Irritation parameter:

other: Eye lense

Time point:

other: after 10 minutes up to 6 days

Reversibility:

not reversible

Remarks:

in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration

Remarks on result:

other: welling up of the eye lenses

Any other information on results incl. tables

The application of the test substance caused severe, irreversible corrosion. After 10 min up to 6 days brown-reddish discoloration of conjunctiva, bloody secretion, opacity and slight chemosis was noted. At the end of the observation period evaluation of the eyes due to suppuration not possible. Welling up of eye lenses.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria