4-[(2,5-dichlorophenyl)azo]-3-hydroxy-N-phenylnaphthalene-2-carboxamide

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

From 2001-01-18 to 2001-05-31

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

1998

Deviations:

no

GLP compliance:

yes

Remarks:

This study was conducted in accordance with "Eco-toxicity test Standards" by Japanese Ministry of the Environment.

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-4-[(2-methyl-5-nitrophenyl)azo]-N-phenylnaphthalene-2-carboxamide

EC Number:

229-245-3

EC Name:

3-hydroxy-4-[(2-methyl-5-nitrophenyl)azo]-N-phenylnaphthalene-2-carboxamide

Cas Number:

6448-95-9

IUPAC Name:

3-hydroxy-4-[(2-methyl-5-nitrophenyl)diazenyl]-N-phenyl-2-naphthamide

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

21 d

Post exposure observation period:

No

Test conditions

Hardness:

Total hardness: 60 to 65 mg/L (as CaCO3)

Test temperature:

Water temperature during 21-day exposure: from 20.0 to 20.2 deg C

pH:

7.9 to 8.2. Thus the variation of pH values was below 1.

Dissolved oxygen:

Dissolved oxygen concentrations were between 7.8 to 8.8 mg/L, thus more than 60% of saturation concentration was maintained in all test groups.

Nominal and measured concentrations:

Nominal test item concentration (limit test): 30 mg/L
Measured test item concentrations were beetween 25 and 34 mg/L (for details see tabel under "Any other information on materials and methods incl. tables". The time weighted mean measured concentration during 21 days was determined to be 30 mg/L.
The concentrations of test substance in the control and solvent control were less than quantification limit (0.06 mg/L).

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 30 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

of parental daphnids - no mortality observed

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 30 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

30 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks:

& parental mortality, macroscopic effects

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 30 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks:

& parental mortality, macroscopic effects

Reported statistics and error estimates:

No-observed-effect concentration (NOEC) and the lowest-observed-effect concentration (LOEC):
After the calculation of the total numbers of juveniles produced per parent alive in each test group for 21 days, the significance (a = 0.05) between exposure group and control group was analyzed by comparing the mean values of these two groups (t-test).
Based on the results, the highest test concentration with no significant difference (no-observed-effect concentration: NOEC) and the lowest test concentration with significant difference (a = 0.05) (lowest-observed-effect concentration (LOEC) compared to the control group were determined.
Initially, a significance test between control and solvent control group was performed by comparing the mean values of these two groups (t-test). Since no
statistically significant difference was determined between these two groups, the value of control group (dilution water only) was used as reference for statistical tests.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes