Registration Dossier
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EC number: 227-930-1 | CAS number: 6041-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- From 2001-01-18 to 2001-05-31
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 1998
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- This study was conducted in accordance with "Eco-toxicity test Standards" by Japanese Ministry of the Environment.
Test material
- Reference substance name:
- 3-hydroxy-4-[(2-methyl-5-nitrophenyl)azo]-N-phenylnaphthalene-2-carboxamide
- EC Number:
- 229-245-3
- EC Name:
- 3-hydroxy-4-[(2-methyl-5-nitrophenyl)azo]-N-phenylnaphthalene-2-carboxamide
- Cas Number:
- 6448-95-9
- IUPAC Name:
- 3-hydroxy-4-[(2-methyl-5-nitrophenyl)diazenyl]-N-phenyl-2-naphthamide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Total hardness: 60 to 65 mg/L (as CaCO3)
- Test temperature:
- Water temperature during 21-day exposure: from 20.0 to 20.2 deg C
- pH:
- 7.9 to 8.2. Thus the variation of pH values was below 1.
- Dissolved oxygen:
- Dissolved oxygen concentrations were between 7.8 to 8.8 mg/L, thus more than 60% of saturation concentration was maintained in all test groups.
- Nominal and measured concentrations:
- Nominal test item concentration (limit test): 30 mg/L
Measured test item concentrations were beetween 25 and 34 mg/L (for details see tabel under "Any other information on materials and methods incl. tables". The time weighted mean measured concentration during 21 days was determined to be 30 mg/L.
The concentrations of test substance in the control and solvent control were less than quantification limit (0.06 mg/L). - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 30 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- of parental daphnids - no mortality observed
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 30 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks:
- & parental mortality, macroscopic effects
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 30 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks:
- & parental mortality, macroscopic effects
- Reported statistics and error estimates:
- No-observed-effect concentration (NOEC) and the lowest-observed-effect concentration (LOEC):
After the calculation of the total numbers of juveniles produced per parent alive in each test group for 21 days, the significance (a = 0.05) between exposure group and control group was analyzed by comparing the mean values of these two groups (t-test).
Based on the results, the highest test concentration with no significant difference (no-observed-effect concentration: NOEC) and the lowest test concentration with significant difference (a = 0.05) (lowest-observed-effect concentration (LOEC) compared to the control group were determined.
Initially, a significance test between control and solvent control group was performed by comparing the mean values of these two groups (t-test). Since no
statistically significant difference was determined between these two groups, the value of control group (dilution water only) was used as reference for statistical tests.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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