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EC number: 212-660-9 | CAS number: 839-90-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented and reported study fully adequate for assessment, although not GLP compliant. The study was conducted according to an internationally accepted technical guideline in a recognized contract research organization.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- of 1983
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tris(2-hydroxyethyl)-1,3,5-triazinetrione
- EC Number:
- 212-660-9
- EC Name:
- Tris(2-hydroxyethyl)-1,3,5-triazinetrione
- Cas Number:
- 839-90-7
- Molecular formula:
- C9H15N3O6
- IUPAC Name:
- tris(2-hydroxyethyl)-1,3,5-triazinane-2,4,6-trione
- Details on test material:
- - Name of test material (as cited in study report): Trishydroxyethylisocyanurat (THEIC)
- Test-substance No.: 87/556
- Degree of purity: ca. 98 %
- Storage: +4°C
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: essential amino acid requiring strains
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver S9 mix from male Sprague Dawley rats treated by single intraperitoneal injection with Aroclor 1254 (500 mg/kg bw) for enzyme induction. Aroclor 1254 was administered 5 days prior to sacrifice.
- Test concentrations with justification for top dose:
- Experiment 1 (Standard Plate Test, without and with metabolic activation (S9 mix), Doses: 0; 20; 100; 500; 2500 and 5000 μg/plate
Experiment 2 (Pre-incubation Test, without and with metabolic activation (S9 mix), Doses: 0; 20; 100; 500; 2500 and 5000 μg/plate - Vehicle / solvent:
- aqua dest.
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- without and with S9 mix
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (disolved in DMSO)
- Remarks:
- Positive control substance for all tests with metabolic activation (S9 mix)
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- without and with S9 mix
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N'-nitro-N-nitrosoguanidine for TA 1535 & TA 100; 4-nitro-o-phenylendiamine for TA 98; 9-aminoacridine chloride monohydrate for TA 1537 (each positive control substance disolved in DMSO)
- Remarks:
- Positive control substances for tests without metabolic activation (S9 mix). All of them are well established reference mutagens.
- Details on test system and experimental conditions:
- Standard Plate Tests were performed in Experiment 1, Pre-incubation Tests in Experiment 2.
Both experiments were conducted without and with metabolic activation (S9 mix)
The following positive controls were used to check mutability of the bacteria and activity of the S9 mix:
With metabolic activation (S9 mix):
2-aminoanthracene:
- 10 μg/plate, dissolved in DMSO: for the strains: TA 1535, TA 100, TA 1537, TA 98
Without metabolic activation (S9 mix):
N-methyl-N'-nitro-N-nitrosoguanidine:
- 5 μg/plate, dissolved in DMSO: for the strains: TA 1535, TA 100
4-nitro-o-phenylendiamine:
- 10 μg/plate, dissolved in DMSO: for the strain: TA 98
9-aminoacridine chloride monohydrate:
- 100 μg/plate, dissolved in DMSO: for the strain: TA 1537 - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control),
- dose-response relationship,
- reproducibility of the results. - Statistics:
- Statistical analysis of the results was not reported. Obviously it was not considered to be necessary.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The test substance was completely soluble in the vehicle, aqua dest.
Contamination was noted only in one plate which was one of the TA 100 without metabolic activation at 20 µg/plate. In all other solvent control, test substance treated or positive control plates contamination was not evident.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results :
negative without and with metabolic activation (S9 mix)
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