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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study under GLP
Remarks:
(as given in OECD SIDS)
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes
Analytical monitoring:
yes
Test organisms (species):
Daphnia magna
Test type:
semi-static
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

- Analytical monitoring: 92-96 % of the nominal concentration at preparation; 93-97 % just before the renewal of the test water.

- Water chemistry in test (O2, pH): DO (mg/L); 8.2-8.9; pH; 7.2-8.4

- EC50: >100 mg/L (21 d reproduction)

- LC50 for parental Daphnia (21 d): >100 mg/L

- NOEC: >=100 mg/L (21 d reproduction)

- Statistical results, as appropriate: There was no statistically significant difference between data from the control and 100 mg/L test groups.

 

Nominal concentrations (as mg/L): 100 

Measured concentrations (as mg/L): 92-97

 

- Table on measured concentrations:

 

Nominal
Concentration

Measured Concentration
(mg/L)

(mg/L)

0 day a

2 days b

8 days a

10 days b

Control

<1

<1

<1

<1

100

93

93

92

97

 

Nominal
Concentration

Measured Concentration
(mg/L)

(mg/L)

20 day a

21 days b

Mean c

Control

<1

<1

<1

100

96

96

94

 

a : freshly prepared test solution

b : old test solution before renewal

c : time-weighted mean

 

- Cumulative numbers of dead parental Daphnia: 

0 % mortality at control and 100 mg/L

- Time of the first brood production of juveniles: 7 d at control and 100 mg/L

 

- Mean cumulative numbers of juveniles produced per live adult: 

____________________________________________________________

 Nominal        Measured      Mean cumulative numbers of 

Concentration  Concentration   juveniles produced 

                                  per live adult  

 (mg/L)         (mg/L)         14 days       21 days

Control            -             59           118

 100            92-97 a         65           118

a : value during the test


- Control response satisfactory: Yes

Conclusions:
There was no statistically significant difference in mean cumulative numbers of juveniles produced per live adult between the treatment group and the control. Hence, 21d-EC50 was greater than 100 mg/L and 21d-NOEC was more than 100 mg/L on chronic Daphnia reproduction test.

Description of key information

NOEC(21 d) ≥100 mg/L for Daphnia magna (OECD TG 211)

Key value for chemical safety assessment

Additional information

Value used for CSA: EC10/LC10 or NOEC for freshwater invertebrates: ≥100 mg/L