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Long-term toxicity to aquatic invertebrates

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Description of key information

EL50, repro; 21d > 10 mg/l (nominal concentration)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
10 mg/L

Additional information

The test item was tested in reproduction test on Daphnia magna. The test was performed according OECD Test Guideline No. 211 -Daphnia magna Reproduction Test. Adopted: October 2, 2012.

Since the test item is a multi-component item the water-accommodated fractions (WAFs) were prepared by adding measured amounts of the test item to the M4 medium and then were stirred for a period of 15 minutes. The preliminary test was performed in a range of the test item loading rates 1 – 10 mg·L-1(loading rate). The test was performed as semi-static, frequency of the test solutions renewal was three times a week. The calculated reduction in reproductive output was 0 % in the highest loading rate, the exact values of EL50could not be calculated on the basis of data acquired. Test result is given in the form of a range. In congruence with study plan, when the assumed value of EL50is higher than 10 mg·L-1, no further testing is needed and the result obtained in preliminary test presents the result of whole study.The experiment with the concentration of 10 mg·L-1included in the preliminary test has been performed using ten replicates for both the treatment and the control groups, therethrough the conditions of the limit testgiven in guidelinehave been fulfilled. 

The analysis for concentration control of the test item was performed by TOC method. For all evaluations and results the loading rates of the test item were used.Under the experimental conditions the EL50, 21d, repro > 10 mg/l (nominal concentration).

For the Chemical safety assessment a value of 10 mg/l is used for calculations