(3-chloro-2-hydroxypropyl)trimethylammonium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: screening tests

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not stated

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The biodegradability of the test substance was studied by adding the material as the sole source of carbon to batch cultures with activated sludge from an industrial waste water treatment plant (AVEBE - Foxhol), which was previously adapted to the substance. Degradation was measured by removal of Dissolved Organic Carbon (DOC) and release of chloride (Cl-). The biological nature of the degradation was confirmed by comparison of the DOC-removal in sterilized batches.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(3-chloro-2-hydroxypropyl)trimethylammonium chloride

EC Number:

222-048-3

EC Name:

(3-chloro-2-hydroxypropyl)trimethylammonium chloride

Cas Number:

3327-22-8

Molecular formula:

C6H15ClNO.Cl

IUPAC Name:

3-chloro-2-hydroxy-N,N,N-trimethylpropan-1-aminium chloride

Details on test material:

Purity: 60% active ingredient in water

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Activated sludge from Foxhol WWTP

- Pretreatment: Washed with mineral medium

- Concentration of organic matter 0.2 g/l

Duration of test (contact time):

10 d

Initial test substance concentration

Initial conc.:

2.5 mmol/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: The mineral medium was prepared by adding 10 ml phosphate solution, 1 ml magnesium sulphate solution, and 1 ml trace solution to 1 litre of demineralised water. The phosphate solution contained 33 g/l (NH4)2HPO4, 43.5 g/l K2HPO4, 54.6 g/l NaH2PO4and 62.3 g/l Na2HPO4. The magnesium sulphate solution contained 246 g/l MgSO4.7H20. The trace solution in 1M HNO3 contained 5.56 g/l FeSO4.7H2O, 1.69 g/l MnSO4.HO0, 2.81 g/l CoSO4.7H2O, 1.47 g/l CaCl2, 0.25 g/l CuSO4.5H2O and 0.29 g/l ZnSo4.7H2O. The medium was sterilised at 120°C in an autoclave.

- Test temperature: 30°C
- pH: 7
- pH adjusted: no

TEST SYSTEM
- Culturing apparatus: Serum bottles (1100 ml) sealed with butyl rubber septum
- Measuring equipment: DOC was measured using a Dohrmann DC-190 TOC analyser
- Test performed in closed vessels due to significant volatility of test substance: No
- Test performed in open system: No


CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes

Reference substance

Reference substance:

not required

Results and discussion

Preliminary study:

No preliminary study.

Test performance:

See details on results

% Degradation

Parameter:

% degradation (DOC removal)

Value:

90

Sampling time:

3 d

Details on results:

Under aerobic conditions 90 % removal of DOC was observed after three days using an initial CHPTAC concentration of 563 mg/l (3
mM). No further degradation was observed from day three till day 10. In the sterile control sample no DOC removal was
observed.

BOD5 / COD results

Results with reference substance:

Not applicable.

Any other information on results incl. tables

In the sterile batches chemical dechlorination was observed; no DOC removal, the chloride concentration increased over the 10 day period from 3.5 mM to 5.3 mM.

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Interpretation of results:

other: under test condition biodegradable

Conclusions:

In a biodegradation test using previously adapted activated sludge, 90% removal of the test substance was observed following 3 days incubation at 30°C. Under the conditions of the test, CHPTAC is therefore considered to be biodegradable. The test does not meet the standards required to assess ready or inherent biodegradation under EC criteria.