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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The notified chemical cannot be classed as readily biodegradable.

Key value for chemical safety assessment

Additional information


OECD Guideline 301B

A modified Sturm test was conducted to determine the ready biodegradability of the test substance in an aerobic, aqueous medium. The extent of biodegradation was determined by monitoring cumulative CO2 production over 28 days for a mineralized inoculum of

microbes in the presence of the test and/or a reference substance.

Inoculum Source:

Sewage treatment plant.

Study Length:

28 days.

Test Concentration:

20 mg carbon/L


The test substance did not biodegrade within a 28- day test period when exposed to microorganisms maintained in an aerobic, aqueous mineralized environment. Cumulative biodegradation only reached 7.27% by day 28.

Cumulative biodegradation of the reference substance (aniline) was 62.8% by Day 14 meeting the test criteria for acceptance.