(2-methoxymethylethoxy)propanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in mammalian cells

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No information is available about GLP, study was conducted equivalent to OECD guideline 476.

Data source

Reference

Reference Type:

publication

Title:

Genotoxic and/or epigenetic effects of some glycol ethers: results of different short-term tests

Author:

Elias, Z. et al. (1996)

Year:

1996

Bibliographic source:

published in Occupational Hygiene, 2: 187-212 (1996).

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)

GLP compliance:

not specified

Type of assay:

mammalian cell gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-methoxypropan-2-ol

EC Number:

203-539-1

EC Name:

1-methoxypropan-2-ol

Cas Number:

107-98-2

Molecular formula:

C4H10O2

IUPAC Name:

1-methoxypropan-2-ol

Details on test material:

- Name of test material (as cited in study report): 2-propylene glycol 1-methyl ether
- Molecular formula (if other than submission substance): C5H13O2
- Molecular weight (if other than submission substance): 90.12
- Isomers composition: 1.2% beta isomer.
- Purity test date: 98.1% purity, 1.2% beta isomer.

Method

Species / strain

Species / strain / cell type:

Chinese hamster lung fibroblasts (V79)

Details on mammalian cell type (if applicable):

- Type and identity of media: DMEM supplemented with 10% fetal calf serum (FCS) ans 2 rnM L-glutamine without antibiotics or fungistatics.
- Properly maintained: yes

Metabolic activation:

without

Test concentrations with justification for top dose:

14-55 mM

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMEM medium

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

methylmethanesulfonate

Evaluation criteria:

There are several criteria for determining a positive result, such as a concentration-related, or a reproducible increase in mutant frequency. Biological relevance of the results should be considered first. Statistical methods may be used as an aid in evaluating the test results. Statistical significance should not be the only determining factor for a positive response. A test substance, for which the results do not meet the above criteria is considered nonmutagenic in this system. Although most studies will give clearly positive or negative results, in rare cases the data set will preclude making a definite judgement about the activity of the test substance. Results may remain equivocal or questionable regardless of the number of times the experiment is repeated.
Positive results for an in vitro mammalian cell gene mutation test indicate that the test substance induces gene mutations in the cultured mammalian cells used. A positive concentrationresponse that is reproducible is most meaningful. Negative results indicate that, under the test conditions, the test substance does not induce gene mutations in the cultured mammalian cells used.

Results and discussion

Test results

Species / strain:

Chinese hamster lung fibroblasts (V79)

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Remarks:

not stated

Remarks on result:

other: other: Chines hamster lung (V79) cells

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Propylene glycol methyl ether was not mutagenic under the conditions of this test.