Benzenesulfonic acid, C10-13-alkyl derivs., calcium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978-05-01 to 1978-08-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Followed guideline, well documented, with analytical.

Justification for type of information:

Study is read-across from LAS. Please refer to section 13 for the read-across justification

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: US EPA, 1975. Reliability, deviations and validity evaluated against the criteria of USEPA 850.1075, 1996.

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

no

Remarks:

study pre-dates GLPs

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Water samples for determination of test concentrations were collected at the beginning and end of each test.
- Sampling method: Samples were preserved with 1% formalin.
- Sample storage conditions before analysis: Not available

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solutions were prepared by diluting the materials in deionized water to the desired concentrations.
- Eluate: Not applicable
- Differential loading: Not applicable
- Controls: Prepared using deionized water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): Not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Common name: Bluegill
- Strain: Not available
- Source: Not available
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): Mean standard length: 4.2 cm, range: 3.0-5.1 cm
- Weight at study initiation (mean and range, SD): Average: 1.1 g, range: 0.5-1.9 g
- Method of breeding: Not available
- Feeding during test: Not fed
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable


ACCLIMATION
- Acclimation period: At least 10 days
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Not available
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available


QUARANTINE (wild caught)
- Duration: Not applicable
- Health/mortality: Not applicable

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Hardness:

Total hardness was 137 mg/L as CaCO3.

Test temperature:

Temperature was maintained at 20 ± 2 °C.

pH:

The pH varied from 7.8 to 8.1.

Dissolved oxygen:

Dissolved oxygen in the fish tests averaged 9.0 (ranged 8.4-9.6) mg/L.

Salinity:

Not applicable

Nominal and measured concentrations:

Details of nominal and measured test concentrations are not available in the study report. Measured concentrations were 79 % of nominal concentrations. Results are based on measured concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed: Not available
- Material, size, headspace, fill volume: 15 liter glass aquaria containing 10 liters of dilution water.
- Aeration: Not available
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Renewal rate of test solution (frequency/flow rate): Not applicable
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.1 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was a blend of well water and deionized water, mixed to the desired hardness.
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: 10 heavy metals were measured, all were <0.2 mg/L. See report.
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: Not available
- Ca/mg ratio: Not available
- Conductivity: Not available
- Culture medium different from test medium: Not available
- Intervals of water quality measurement: Dissolved oxygen, pH and hardness were monitored twice during the test.


OTHER TEST CONDITIONS
- Adjustment of pH: Not applicable
- Photoperiod: 16 hours illumination
- Light intensity: Not available


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Fish mortality recorded 1, 3 and 5 hours after test initiation, and daily until test termination.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: Not available
- Justification for using less concentrations than requested by guideline: Not applicable
- Range finding study: No

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.67 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 1.58-1.77mg/L

Details on results:

- Behavioural abnormalities: None
- Observations on body length and weight: None
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Probit analysis of all mortality data in this study followed the procedures of Finney (1971).

Validity criteria fulfilled:

yes

Remarks:

All the criteria of USEPA OPPTS 850.1075, 1996 were met

Conclusions:

The 96 h LC50 of C11.8 LAS (linear alkylbenzene sulfonate) to bluegill (Lepomis macrochirus) was 1.67 mg/L, based on mean measured concentrations of active ingredient.

Executive summary:

In a 96 h acute toxicity study, Bluegill (Lepomis macrochirus) were exposed to the read-across source substance C11.8 LAS (linear alkylbenzene sulfonate, an anionic surfactant) under static conditions. Nominal concentrations were not specified in the report. Measured concentrations were 79 % of nominal concentrations.

The 96 h LC50 value, based on measured concentration, was 1.67 mg a.i./L, with 95% CL 1.58 to 1.77 mg/L.

This toxicity study is classified as acceptable and satisfies the guideline requirement for the current USEPA OPPTS 850.1075 (1996) acute fish toxicity study.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study done according to OECD guideline 203, however, only minimal information reported.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test type:

static

Limit test:

no

Total exposure duration:

96 h

Nominal and measured concentrations:

0, 1, 10, 100 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 - < 10 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Conclusions:

The 96-hr LC50 in fish is >1-<10 mg/L.

Executive summary:

Fish were exposed to concentrations of 0, 1, 10, or 100 mg/L for 96 hrs. The LC50 was >1 -<10 mg/L.

Description of key information

In the key 96 h acute toxicity study, Bluegill (Lepomis macrochirus) were exposed to the source analogue substance C11.8 LAS (linear alkylbenzene sulfonate, an anionic surfactant) under static conditions. Nominal concentrations were not specified in the report. Measured concentrations were 79 % of nominal concentrations.

The 96 h LC50 value, based on measured concentration, was 1.67 mg a.i./L, with 95% CL 1.58 to 1.77 mg/L.

This toxicity study is classified as acceptable and satisfies the guideline requirement for the current USEPA OPPTS 850.1075 (1996) acute fish toxicity study.

In another study on the target (registration) substance, the 96-hr LC50 in fish was determined to be >1-<10 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 mg/L

Additional information

In a supporting study on the target (registration) substance, fish were exposed to concentrations of 0, 1, 10, or 100 mg/L for 96 hrs. The LC50 was >1 -<10 mg/L. The lowest value in the range was selected as the key value for the CSA. This is a conservative choice as the LC50 value of 1 mg/l is lower than the LC50 value of 1.67 mg/L obtained for the read-across source substance LAS.