Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

toxicity to terrestrial plants
Type of information:
experimental study
Adequacy of study:
supporting study
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: According to EU RAR 2005 no EC50 or NOEC can be derived from this study.

Data source

Referenceopen allclose all

Reference Type:
Effect, uptake and disposition of nitrobenzene in several terrestrial plants
McFarlane C, Pfleeger T
Bibliographic source:
Environ Tox Chem 9: 513 - 520
Reference Type:
secondary source
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
European Chemicals Bureau
Bibliographic source:
3rd Priority List, Volume: 77
Report date:

Materials and methods

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:

Sampling and analysis

Analytical monitoring:
not specified

Results and discussion

Any other information on results incl. tables

In a 72 hours phytotoxicity test eight species of plants were exposed to nitrobenzene in exposure chambers (McFarlane et al., 1990). All plant species examined in this study were provided with nutrient medium containing nitrobenzene. Phytotoxicity to nitrobenzene varies considerably between species. When roots were dosed at 8 mg/l the photosynthesis and transpiration responses vary from no effect to complete suppression. No visible symptoms or changes in the transpiration or photosynthetic rates occurred with soybeans (glycine maximus), barley (hordeum vulgare), honeysuckle (lonicera tatarica) and poplar (populus robusta). For these species the 72 h NOEC ≥ 8 mg/l. Green ash (fraxinus pennsylvanica) and lettuce (lactuca sativa) showed no visible symptoms but suffered an initial decrease in both transpiration and photosynthesis rate. The ash plants started recovery after about 10 hours. Lettuce plants recovered much more slowly, the photosynthetic rate started to increase after about 60 hours. Two Elaeagnus species seem to be the most sensitive to nitrobenzene. Autumn olive (Elaeagnus umbellata) did not survive the dosing of 8 mg/l (LC100 = 8 mg/l). Shortly after dosing the transpiration and photosynthetic rate decreased rapidly and did not recover, leaves dropped spontaneously and by the end of the study all remaining leaves dropped when the plants were touched. Russian olive plants (Elaeagnus angustifolia) were similar to the autumn olive in that some of the leaves on some of the plants dropped. However, the newest leaves and all leaves on one plant remained intact and continued to function (photosynthesis and transpiration), although at reduced rates. Recovery started after about 10 hours and was complete at the end of the experiment. Inhibition of root growth of soybean plants (glycine maximus) without an accompanied impairment of transpiration and photosynthesis rate was also observed (Fletcher J et al., 1990). The plants were exposed to nitrobenzene concentrations of 0.02 to 100 mg/l via roots and harvested after 72 hours. The lower concentration of nitrobenzene did not appear to cause plant damage or alter shoot growth. But a visual examination of roots before and after nitrobenzene exposure indicated that the highest concentration inhibited root growth.

Applicant's summary and conclusion