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EC number: 931-274-8 | CAS number: 28182-81-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial radical formation potential
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- (1987)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 28182-81-2
- Cas Number:
- 28182-81-2
- IUPAC Name:
- 28182-81-2
- Reference substance name:
- HDI oligomers, isocyanurate
- EC Number:
- 931-274-8
- Cas Number:
- 28182-81-2
- Molecular formula:
- (C8H12N2O2)n
- IUPAC Name:
- HDI oligomers, isocyanurate
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Regal Group UK, Great Bookham, Surrey, UK.
- Age at study initiation: approx. 14 to 17 weeks
- Weight at study initiation: weight range of 3.4 - 3.8 kg
- Housing: individually in metal cages with perforated floors
- Diet and water: ad libitum
- Acclimation period: All rabbits were acclimatised to the experimental environment. No information concerning period of time.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18-19.5
- Humidity (%): 44-76
- Air changes (per hr): approx. 19
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the contralateral eye remained untreated and served as control
- Amount / concentration applied:
- 0.1 mL
- Duration of treatment / exposure:
- The eyelids were gently held together for one second before releasing.
- Observation period (in vivo):
- Examinations were terminated after 7 days, since all changes had completely subsided then.
- Number of animals or in vitro replicates:
- 3 (two males, one female)
- Details on study design:
- PROCEDURE: The eyes of each animal were examined prior to instillation of the test substance to ensure that there was no pre-existing corneal damage, iridial or conjunctival inflammation.
One animal was treated in advance of the others, to ensure that if a severe response was produced, no further animals would be exposed. Approximately 0.1 mL of the test substance was placed into the lower everted lid of one eye of each animal. The eyelids were gently held together for one second before releasing.
SCORING SYSTEM:
Cornea opacity - degree of density: No ulceration or opacity 0; Scattered or diffuse areas of opacity (other than slight dulling of normal lustre), details of iris clearly visible 1; Easily discernible translucent areas, details of iris slightly obscured 2; Nacreous areas, no details of iris visible, size of pupil barely discernible 3; Opaque cornea, iris not discernible through the opacity 4
Iris: Normal 0; Markedly deepened rugae, congestion, swelling, moderate circumcorneal hyperaemia or injection, any of these or combination of any thereof, iris still reacting to light (sluggish reaction is positive) 1; No reaction to light, haemorrhage, gross destruction (any or all of these) 2
Conjunctivae redness: Blood vessels normal 0; Some blood vessels definitely hyperaemic (injected) 1; Diffuse, crimson colour, individual vessels not easily discernible 2; Diffuse beefy red 3
Chemosis: No swelling 0; Any swelling above normal (includes nictating membranes) 1; Obvious swelling with partial eversion of lids 2; Swelling with lids about half-closed 3; Swelling with lids more than half-closed 4
TOOL USED TO ASSESS SCORE: Observation of the eyes was aided by the use of a handheld light.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: #1, #2, #3
- Time point:
- other: 24, 48, 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- iris score
- Basis:
- animal: #1, #2, #3
- Time point:
- other: 24, 48, 72 hours
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- conjunctivae score
- Remarks:
- (redness)
- Basis:
- animal #1
- Time point:
- other: 24, 48, 72 hours
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- conjunctivae score
- Remarks:
- (redness)
- Basis:
- animal: #2, #3
- Time point:
- other: 24, 48, 72 hours
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 4 days
- Irritation parameter:
- chemosis score
- Basis:
- animal: #1, #3
- Time point:
- other: 24, 48, 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- other: 24, 48, 72 hours
- Score:
- 0 - 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 3 days
- Remarks on result:
- other: mean score: 0.7
- Irritant / corrosive response data:
- No corneal damage or iridial inflammation was observed. Hyperaemia of blood vessels with slight swelling was seen in all animals. These reactions had resolved in all instances by four or seven days after instillation.
- Other effects:
- There were no signs of toxicity or ill health in any rabbit during the observation period.
Applicant's summary and conclusion
- Executive summary:
An Acute Eye Irritation/Corrosion test was conducted according to OECD TG 405. In this study no corneal damage or iridial inflammation was observed (all scores zero). Hyperaemia of blood vessels with slight swelling was seen in all animals (Mean scores for time points 24, 48, and 72 h: 1 for conjuntiva/redness, 0.2 for chemosis). These reactions had resolved in all instances by four or seven days after instillation. No signs of toxicity or ill health were observed.
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