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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Not all information necessary is given, no results per concentration given, no analytical monitoring and no GLP. Despite these shortcomings, the results can be used.
Principles of method if other than guideline:
Method: other: artificial soil test, laboratory test of the BBA. Resembles OECD Guideline 207
GLP compliance:
Analytical monitoring:
Test organisms (species):
Eisenia fetida
Study type:
laboratory study
Substrate type:
other: artificial soil test
Limit test:
Total exposure duration:
14 d
14 d
Dose descriptor:
Effect conc.:
> 750 mg/kg soil dw
Nominal / measured:
Conc. based on:
act. ingr.
Basis for effect:
- Nominal concentrations: 0, 7.5, 37.5, 75, 375 and 750 mg
a.i./kg dw
- Effect data:
Weight: For all concentrations the weight of the worms was
approx. 90-95% of the control.
Mortality: No information given on mortality per test
- LC50 > 750 mg a.i./kg dw

Description of key information

The LC50 for soil dwelling organisms is greater than 750 mg/kg soil dw.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
750 mg/kg soil dw

Additional information

One study was found valid without restrictions. In that study performed with Eisenia fetida in artificial soil according to OECD 207 and under GLP no mortality or weight loss was observed (Winkelmann, 2006). Furthermore, two studies were found valid with restrictions. In the study performed by Haque and Ebing (1983) two worm species were tested Eisenia fetida and Lumbricus terrestris L.. For both species 50% effect was not reached. The other study performed by Bouche and Beugnot (1978) was a field study. Two parcels of land (52x36.2 m) were at first exposed to sodium chlorate at a concentration of 200 kg/ha, about two and a half months later a higher concentration of 400 kg/ha was applied. Control parcels were not exposed. One further parcel was simultaneously exposed to chlordane. Six earthworm species were recovered from control treated parcels and the first application did not result in an effect on any of the species found, there was no significant difference found in number and biomass of earthworms per species compared to the control field. An effect was found one week after the second application of 400 kg/ha. The number and biomass of the earthworm populations reduced compared to the control, but within one month complete recovery was shown.

The LC50 for soil dwelling organisms is greater than 750 mg/kg soil dw.