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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 June 1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
1984
Deviations:
yes
Remarks:
- Sludge from a Husman laboratory apparatus
Qualifier:
according to guideline
Guideline:
other: ETAD method No. 103
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Test substance: FAT-40'400/A
Stability: guaranteed by the sponsor
Solubility: ~ 100 g/l
Storage: room temperature
Appearance: solid
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: Mixed culture of bacteria: Sludge of a Husman apparatus
Details on inoculum:
Laboratory culture: Mixed culture of bacteria: Sludge of a Husman apparatus.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
21.3°C
Nominal and measured concentrations:
Nominal concentrations: 1, 3.2, 10, 32 and 100 mg/L
Details on test conditions:
- Temperature: 21.3°C
- Duration: 3 h
- Estimation technique: Oxygen consumption measured with an oxygen sensitive electrode system: OXY DIGI 550.
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The IC50 (3 h) of FAT-40'400/A is > 100 mg/l.
Results with reference substance (positive control):
The IC50 of the reference substance 3,5-Dichlorophenol is: 19 mg/L (Criteria: 10 - 25 mg/L)
Validity criteria fulfilled:
yes
Conclusions:
IC50 for FAT 40400/A >100 mg/L.
Executive summary:

The IC50 of the test substance was determined in a respiration inhibition test performed according to OECD TEST Method 209, April 1984, corresponding to ETAD-Method No.103 under GLP. In this study, a mixed culture of bacteria (sludge of a Husman apparatus) was exposed to 1, 3.2, 10, 32 and 100 mg of test substance/L for 3 hours. The test substance had no significant inhibitory effect (<15%) on the respiration rate of activated sludge after the incubation period of 3 hours, on the test conditions. Based on the study results, IC50 was estimated to be >100 mg/L.

Description of key information

The IC50 of the test substance was determined in a respiration inhibition test performed according to OECD TEST Method 209, April 1984, corresponding to ETAD-Method No.103 under GLP. In this study, a mixed culture of bacteria (sludge of a Husman apparatus) was exposed to 1, 3.2, 10, 32 and 100 mg of test substance/L for 3 hours. The test substance had no significant inhibitory effect (<15 %) on the respiration rate of activated sludge after the incubation period of 3 hours, on the test conditions. Based on the study results, IC50 was estimated to be >100 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L

Additional information