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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
A mixture of: 2,2,6,6-tetramethylpiperidin-4-yl-hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl-octadecanoate
EC Number:
415-430-8
EC Name:
A mixture of: 2,2,6,6-tetramethylpiperidin-4-yl-hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl-octadecanoate
Cas Number:
86403-32-9
Molecular formula:
CnH2n+2O2, where n=12 to 18
IUPAC Name:
2,2,6,6-tetramethylpiperidin-4-yl hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl octadecanoate

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain/clone:Straus
- Justification for species other than prescribed by test guideline:
- Source: laboratory own culture
- Age of parental stock (mean and range, SD): 24 h
- Feeding during test: yes
- Food type: Chlorococcal Alga
- Amount: 0.1 to 0.2 mg/L TOC
- Frequency:1/day

ACCLIMATION
- Acclimation period:4 week
- Acclimation conditions (same as test or not):not
- Type and amount of food:Chlorococcal Alga
- Feeding frequency:1/day
- Health during acclimation (any mortality observed): no


Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
21 d

Test conditions

Hardness:
> 140 mg/L
Test temperature:
20 °C ± 2°C, constant in this range ±1°C
pH:
6-9, it should not change by more than 1.5 during test
Dissolved oxygen:
> 3 mg/L, withouth aeration
Nominal and measured concentrations:
nominal: 0.054 , 0.081, 0.12, 0.18, 0.27, mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:glass vessels
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 150 ml
- Aeration:no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates):10
- No. of vessels per vehicle control (replicates):0




OTHER TEST CONDITIONS
- Adjustment of pH: yes, at the start and in the end
- Photoperiod:16 h light/ 8h dark
- Light intensity: 600 - 800 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, , number of living juveniles, pH, dissolved oxygen conc., temperature, light intensity, water hardness, TOC, test substance stability control

VEHICLE CONTROL PERFORMED: no

RANGE-FINDING STUDY
- Test concentrations:0.054 -0.27 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
0.19 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95 %CL
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.054 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
0.081 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality of parent animals: 10%
- No. of offspring produced per day per female:

- Number of males and females (parental): no males
- Time to first brood release or time to hatch: 6th day

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
As already mentioned above, all test conditions were fulfilled during the performed water flea Daphnia magna reproduction study.
10% mortality of parential females of water flea Daphnia magna in the control group was recorded. The first offspring in the control appeared as soon as on day 6 (i.e.before day 11), the mean number of surviving juveniles per parent organism in the control was 138. Variation coefficient of the number of surviving juveniles per parent individual in the control=10.9% and therefore the results of the water flea Daphnia magna reproduction toxicity test are considered valid. According to the results of the water flea Daphnia magna reproduction toxicity test the following value were determined for the test substance DASTIB 845:
21d EC50= 0.19 mg/L
NOEC = 0.054 mg/L
LOEC = 0.081 mg/L