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Toxicity to aquatic algae and cyanobacteria

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toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 1987-March 1991
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets National standards method with acceptable resctrictions.
according to guideline
other: EPA TSCA Experimental Method 797.1060 (U.S.EPA, 1985&1986)
GLP compliance:
not specified
Analytical monitoring:
Details on sampling:
- The stability of the NG stock solution were periodically checked during storage to assure that no decomposition occured. Standard solutions of NG were prepared by dilution of the stock solution freshly prepared each day of analysis. Standard solutions with concentrations of 10.0, 5.0, 2.5, and 1.0 µl/L were used.- Aqueous samples from bioassays were injected directly into HPLC after filtration to remove particles > 0,45 µm.In the cases where samples could not be analysed immediately following filtration, the filtered samples were stored at 4°C in amber glass vials fitted with Teflon-lined caps and analyzed within 24 h from the time the samples were originally taken from the test aquaria.
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION - Basic solution (the shipment): 1 g NG dissolved in JHU/APL well water. NG solutions were keep in the dark at 4°C in glass bottles.- NG stock solutions were prepared by adding appropriate amounts of the material to aerated JHU/APL well water and stirred for 4 to 8 h. No NG stock solutions were heated during preparation or filtering at 0.45 µm. All stock solutions were prepared in amber glass containers.
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM- Source (laboratory, culture collection): The culture collection at North Texas State University, Denton, TX. - Stock algal cultures were reared in 2.5L Pyrex® culture flasks containing 1L of filter sterilized double strength "AAP" algal assay medium. Cultures were maintained in a constant temperature incubator under constant cool-white fluorescent lights (≈300 foot candles) at a temperature of 20 (± 0.4)°C. on a shaker table oscillating at 100 rpm (± 10). Temperatures of the baths, test chambers, and the control chamber were monitored continuously and recorded continuously on strip charts. Cells in the log growth phase were used to start all tests.
Test type:
Water media type:
Total exposure duration:
96 h
Test temperature:
20.1°C (19.7-20.4°C)
Nominal and measured concentrations:
Nominal concentration: 0.22, 0.44, 0.72, 1.20, 2.00 mg/LMeasured concentration: 0.18, 0.37, 0.59, 1.14, 1.89 mg/L
Details on test conditions:
TEST SYSTEM- Test vessel: 250 mL Delong flask- Fill volume: 0.1 L- Initial cells density: 4000 cells/mL- No. of vessels per concentration (replicates): 3- No. of vessels per control (replicates): 3- No. of vessels per vehicle control (replicates): 3GROWTH MEDIUM- Medium used: The nutrient media sterilized double strenght "AAP" algal assay medium, with sufficient P added to achieve a 20:1 N:P ratio as described in Miller at el. (1978)TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Non-chlorinated deep well located at The Johns Hopkins University Applied Physics Laboratory (JHU/APL) in Shady Side, MD.- Total organic carbon: 19 mg/L- Metals: Sb, As, Be, Pb, se, Tl < 0.005 mg/L, Cd < 0.001 mg/L, Cr < 0.05 mg/L, Cu < 0.02 mg/L, Hg < 0.0002 mg/L, Ni < 0.2 mg/L, Ag <0.01 mg/L, Zn < 0.04 mg/L- Alkalinity: 156 mg/L as CaCO3- - The water used in was analyzed for 43 Priority Pollutants, eight nonpriority, but "Hazardous" substances, 26 pesticides, and 13 metals. None of them were detected with detection limits of 2, 2, 0.1 µg/, and <0.2 - <0.0002 mg/, respectively.- Intervals of water quality measurement: A comprehensive chemical analysis of the well water was conducted two times during the study. The analyses were separated by approx. 18 months.OTHER TEST CONDITIONS- Photoperiod: constant- Light intensity and quality: cool-white fluorescent lights (≈300 foot candels)EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :- Determination of cell concentrations: Model ZBI Coulter Counter (Coulter Electronics Inc., Hialeah, FL)TEST CONCENTRATIONS- Spacing factor for test concentrations: 0.6
96 h
Dose descriptor:
Effect conc.:
1.15 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
cell number
Remarks on result:
other: 0.8-2.06 mg/L
Reported statistics and error estimates:
The 96 hr. EC50 values for growth inhibition were calculated using the "inhibition proportion" technique recommended by Horning & Weber (1985). Because of the very nature of the growth data, the assumptions of the probit analysis were not met in the classical sense. Therefore, the count data were averaged and subsequently converted to "inhibition proportions" using the following formula. I = C – (T/C) x 100, where C = mean growth of controls; and T = mean growth at the given treatment. The probit analysis was then performed on these "inhibition proportions".
The 96 hr EC50 for TNG for S.Capricornutum (based on cell density) was 1.15. mg/L.When the data are treated as chronic data rather than acute, the LOEC and NOEC for growth are 0.59 and 0.37 mg/L, respectively.

Description of key information

The contact of the nitroglycerine with the marine water is impossible in normal conditions of its production and using of explosives.

Key value for chemical safety assessment

EC50 for freshwater algae:
1.15 mg/L

Additional information