1,1',1''-nitrilotripropan-2-ol

Long-term toxicity to aquatic invertebrates

Description of key information

With high probability long-term effects on aquatic invertebrates are not expected (NOEC >= 10 mg/L).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

>= 10 mg/L

Additional information

The long-term effects of the substance on Daphnia magna were investigated in GLP study following OECD TG 211 (Isopropanol Amines Consortium, 2022; report no. S21-05542).

A range finder showing no adverse effects at the highest test concentration of 10 mg/L was done beforehand. As no adverse effects occured, the final test was conducted as a limit test at 10 mg/L in accordance with OECD guideline 211, section 36. As a “safety net approach” (to meet potential toxicity discrepancies between the range finder and the final toxicity test) 1.0 mg/L and 3.16 mg/L were additionally set up in the final toxicity test.

The test was conducted semi-static with nominal concentrations of 1.00, 3.16 and 10.0 mg/L and control with renewal of test solutions three times per week. The test item concentrations were analytically verified and proved to be stable (94 to 107% of nominal values); therefore, the effect values are expressed as nominal concentrations. The validity criteria of the guideline were met. The study is valid.

The first offspring was observed on day 7 in the control and all treatments. No parental mortality occurred over the complete exposure period in the control and all treatments. Parental mortality and alive offspring per alive adult per test end were evaluated: The 21-d LOEC values are > 10.0 mg/L (nominal). The NOEC was observed at >= 10.0 mg/L  (nominal).

It can be concluded that with high probability long-term effects on aquatic invertebrates are not expected (NOEC >= 10 mg/L).