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Short-term and long-Toxicity to Fish

The acute and chronic studies with benzyl acetate were carried out under flow-through test conditions, test species was medaka (Oryzias latipes). The 96 hour LC50 was calculated to be 4 mg/L (with 95% confidence limits of 3.48 – 4.60mg/L). The chronic value was calculated to be 1.33 mg/L (Holcombeet al, 1995).

Short-term Toxicity to Aquatic Invertebrates

The acute toxicity of the benzyl acetate to the freshwater invertebrate Daphnia magna was investigated . The 48-hour EC50 was 17 mg/L (95 % confidence limits 14 -20 mg/L), the corresponding no-observed effect concentration was 10 mg/L (Vyrenhoef, H. and Mullee, D.M. 2010).

Toxicity to Aquatic Algae

The effects of benzyl acetate on the growth of the alga Desmodesmus subspicatus were investigated (Vyrenhoef, H. and Mullee, D.M. 2010). Growth rate: 72 h EC50: 110 mg/L; NOEC: 52 mg/L; LOEC: 113 mg/L Yield: 72 h EC50: 92 mg/L; NOEC: 52 mg/L; LOEC: 113 mg/L.

Toxicity to microorganisms

A study was performed to assess the effects of benzyl acetate on microorganisms. The method followed that described in the ISO 8192-1986 requirement E guideline (equivalent to OECD 209) (Muller, 1994).

Nominal exposure concentrations were 180, 320, 560, 1000 and 1800 mg/L.  Respiration rates were 33.0, 32.0, 25.5, 11.0 and 3.0 mg/L.h and the % inhibition values were 0, 0.9, 21.1, 65.9 and 90.7%. The EC50 was 855 mg/L.

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