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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Justification for Read across to other Glymes

Monoglyme (CAS No. 110 -71 -4 ), Diglyme (CAS No. 111 -96 -6) and Triglyme (CAS No. 112 -49 -2) are already registered under REACH. These substances are chain homologues of the registration substance Tetraglyme (CAS No. 143 -24 -8). All these Glymes which differ in their structure by the number of the repeating group OCH2CH2 (n=1 -4) have very low acute and chronic ecotoxicity which are in most cases in the same order of magnitude. For Tetraglyme the acute and chronic data from Di- and Triglyme were used for read across.To confirm acceptability of the read across acute daphnia and algae tests were carried out for Tetraglyme. In the table below all available acute an chronic ecotoxicity studies for Glymes are summarized to demonstrate the applicability of the read across.


Acute FishOECD 203LC50 (96h)>5000 (Tri-G)>5000 (Tri-G)>5000>5000 (Tri-G)
Acute DaphniaOECD 202EC50 (48h)4000943>50007467
AlgaeOECD 201ErC50 (72h)9120>10,000>60008996
MicroorganismsOECD 209EC50 (3h)>6400>5000>5000>5000 (Tri-G)
  NOEC (3h)2001067>5000>5000 (Tri-G)
Chronic DaphniaOECD 211NOEC (21d)320 (Di-G)320320 (Di-G)320 (Di-G)
AlgaeOECD 201ErC10 (72h)3793831830002871


(Di-G) means read across from Digylme, (TriG) means read across from Triglyme



For Tetraglyme short-term aquatic ecotoxicity studies for all three trophic levels (fish, daphnia and algae) are available as well as studies on sludge respiration inhibition.


 Fish: OECD 203  LC50 (96h)  > 5000 mg/L  Read across Tri-G
 Daphnia: OECD 202 EC50 (48h)  5000 mg/L Key Study
 Algae: OECD 201 ErC50 (72h)  8996 mg/L Key Study
 Microorganisms (Sludge) OECD 209 NOEC (3h) > 5000 mg/L Read across Tri-G
   EC50 (3h) > 5000 mg/L Read across Tri-G

Tri-G means Trigylme


The short-term ecotoxicity data were used for the derivation of the PNEC aqua intermittent release (see Section 6 Tetraglyme PNEC).



Read across from Diglyme 21d Daphnia study

For reasons given in the waiving statements Section 6.1.2 no long-term fish toxicity tests were carried out. Instead a Diglyme long-term invertebrate study with daphnia was established. This 21d daphnia study with Diglyme (REACH Registration No. 01-2119485900-34-0000) was used for read across to Tetraglyme (see Section 6.1.4) for justification.


Algae:OECD 201  ErC10 (72h)2871 mg/L  Key Study
 Daphnia: OECD 211 NOEC (21d)  320 mg/L Read Across from Diglyme


The algae ErC10 (72h) and the daphnia NOEC (21d) were used to derive the PNEC aqua for continous release (see Section 6 Tetraglyme PNEC).