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The 72h-EC50 for growth rate inhibition (ERC50) and yield inhibition (EYC50) exceeded a
loading rate of 6.3 μg/L. The 72h-NOEC for both growth rate inhibition and yield inhibition was 6.3 μg/L.
Due to the very low solubility of Pigment Orange 5 in water, concentration levels that might be toxic for algae could not be reached.


In the acute Daphnia study, C.I. Pigment Orange 5 showed no biologically significant effects at the saturated solution of the test item. Therefore, The EC50 (48h( was determined to be higher than the concentration of a saturated solution.

In the long-term Daphnia reproduction study, Pigment Orange 5 did not affect reproduction, growth and survival of Daphnia magna at the highest tested concentration of 6.3 µg/L after 21 days of exposure (NOEC).

 Long term Fish:

NOEC (embryonic survival, post-hatch survival, body weight, body length) ≥ 6.3 μg/L, based on analytically confirmed nominal concentrations.



Additional information

No toxic effect was observed at the water solubility (6.3 μg/L) of the test substance at any of the aquatic toxicity tests.