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EC number: 203-312-7 | CAS number: 105-59-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 997
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Principles of method if other than guideline:
- Method according to Schmid (1975) and McGregor (1980)
- GLP compliance:
- not specified
- Type of assay:
- mammalian erythrocyte micronucleus test
Test material
- Reference substance name:
- 2,2'-methyliminodiethanol
- EC Number:
- 203-312-7
- EC Name:
- 2,2'-methyliminodiethanol
- Cas Number:
- 105-59-9
- Molecular formula:
- C5H13NO2
- IUPAC Name:
- 2-[(2-hydroxyethyl)(methyl)amino]ethan-1-ol
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss Webster
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 6-8 weeks
Administration / exposure
- Route of administration:
- intraperitoneal
- Duration of treatment / exposure:
- single application
- Frequency of treatment:
- once
- Post exposure period:
- 30, 48 and 72 hours
Doses / concentrationsopen allclose all
- Dose / conc.:
- 175 mg/kg bw/day (nominal)
- Dose / conc.:
- 350 mg/kg bw/day (nominal)
- Dose / conc.:
- 560 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- triethylenemelamine
- Route of administration: i.p.
- Doses / concentrations: 0.3 mg/kg
- 30 h post dosing
Examinations
- Tissues and cell types examined:
- peripheral blood erythrocytes
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION:
based on pretest: about 80, 50 and 25 % of the LD50
DETAILS OF SLIDE PREPARATION:
Slides of blood smears were stained with Gurr's R-66 Giemsa diluted in phosphate buffer, coded and read without knowledge of treatment group to prevent bias.
METHOD OF ANALYSIS:
The polychromatic/normochromatic erythrocyte ratio for approximately 1000 total cells for each animal was calculated to provide an estimate of cytotoxicity.
- Evaluation criteria:
- A positive result was concluded if at least one statistical significant increase above vehicle control was an indication of a dose-related effect.
- Statistics:
- Data were compared for significant differences using the Fisher's Exact Test.
Results and discussion
Test results
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- There were no significant differences in the polychromatic erythrocyte to normochromatic erythrocyte ratios at any dosage. Furthermore, no significant increases in the incidence of micronucleated polychromatic erythrocyte were observed at any sampling time. Therefore, MDEA is not considered to be inducer of micronuclei under the condition of this in vivo test.
Any other information on results incl. tables
Induction of micronucleus in peripheral erythrocytes:
Sex | Mean PCE/1000 NCE (±SD) | ||||
Water | TEM | MDEA (mg/kg) | |||
175 | 350 | 560 | |||
30 h postdosing | |||||
Male | 52±24 | 36±8 | 55±17 | 64±11 | 70±12 |
Female | 35±10 | 36±9 | 41±8 | 48±9 | 41±14 |
48 h postdosing | |||||
Male | 43±13 | 45±17 | 43±12 | 36±4 | |
Female | 31±6 | 34±18 | 32±12 | 38±4 | |
72 h postdosing | |||||
Male | 39±7 | 38±12 | 36±14 | 32±14 | |
Female | 32±9 | 31±11 | 39±9 | 24±7 |
Sex | Mean MN-PCE/1000 NCE (±SD) | ||||
Water | TEM | MDEA (mg/kg) | |||
175 | 350 | 560 | |||
30 h postdosing | |||||
Male | 5.4±3.2 | 42.0±8 b | 4.8±2.4 | 6.6±2.9 | 4.8±3.5 |
Female | 2.6±1.7 | 43±15 b | 4.2±2.8 | 3.0±0.7 | 1.6±1.5 |
48 h postdosing | |||||
Male | 3.8±3.1 | 4.2±2.8 | 5.0±4.4 | 3.0±2.0 | |
Female | 2.4±1.5 | 3.8±2.4 | 2.2±1.3 | 2.6±2.7 | |
72 h postdosing | |||||
Male | 5.0±2.0 | 2.6±2.1 | 7.0±5.6 | 2.7±1.2 | |
Female | 3.2±1.1 | 2.2±1.3 | 3.0±2.8 | 2.8±1.9 |
There were no significant differences in the polychromatic erythrocyte to normochromatic erythrocyte ratios at any dosage. Furthermore, no significant increases in the incidence of micronucleated polychromatic erythrocyte were observed at any sampling time. Therefore, MDEA is not considered to be inducer of micronuclei under the condition of this in vivo test.
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