Leach residues, zinc ore-calcine, zinc cobalt

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: read accross of critical study record from specific substance (CdCl2) driving classification

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication that meets basic scientifc principles, similar to standard

Data source

Reference

Reference Type:

publication

Title:

Differences in acute toxicity test results of three reference toxicants on Daphnia at two temperatures

Author:

Lewis PA and Horning II WB

Year:

1991

Bibliographic source:

Environmental Toxicology and Chemistry, 10, 1351-1357.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA 600/4-78 012

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cadmium chloride

EC Number:

233-296-7

EC Name:

Cadmium chloride

Cas Number:

10108-64-2

IUPAC Name:

cadmium dichloride

Details on test material:

CdCl2

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Stock solution of 72mg/L Cd as CdCl2 was prepared and kept in the refrigerator

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation (mean and range, SD): neonates
- Feeding during test
- Food type: fish food solution
- Amount: 0.3ml/vessel
- Frequency: once at beginning of test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

160-180 mg CaCO3/L

Test temperature:

20°C

pH:

8.5

Nominal and measured concentrations:

control and 3, 9, 27, 81, 243 and 729 µg Cd/L; 10 replicates
nominal equivalenty to measured concentrations

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

38 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mortality

Reported statistics and error estimates:

LC50 and confidence limits were calculated by moving average angle method

Any other information on results incl. tables

none

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Tests done according to a standard protocol thus of good quality and considered useful for setting the reference value for acute aquatic ecotoxicity.

Executive summary:

Tests done according to a standard protocol, of good quality and considered useful for setting the reference value for acute aquatic ecotoxicity. Study used for that purpose in the EU risk assessment process on cadmium (ECB 2008). Culture medium, test water conditions well described and relevant the purpose of classification.