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The effects of isophorone diisocyanate on the inhibition of the respiration rate of activated sludge ( from a STP treating predominantly domestic sewage) was investigated by Bayer AG (2000) according to EU method C.11. The oxygen content was measured at five concentrations ranging from 100 to 1000 mg/L over a period of 3 hours. The EC50 (3 hours contact time) was determined as 263 mg/L.

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