Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl cyanoacetate
EC Number:
203-309-0
EC Name:
Ethyl cyanoacetate
Cas Number:
105-56-6
Molecular formula:
C5H7NO2
IUPAC Name:
ethyl 2-cyanoacetate
Details on test material:
Batch no. Tank 434
ID no. 3633/81410

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus, strain IRCHA
max. age 24 hours

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 ± 1°C
pH:
6 - 7.9 pH
Dissolved oxygen:
4.8 - 8.1 mg/l
Salinity:
2.5 mmol/L Ca/Mg ions proportion 4:1
Nominal and measured concentrations:
100, 140, 200, 280, 400, 560 and 800 mg/l
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
613 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
471 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
After an 24 hour exposure with potassium dichromate in concentrations of 0.9 and 1.9 mg/l, the immobility was at 0.9 mg/l 30% and at 1.9 mg/l 100%.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of Ethylcyanoacetat at 24 h is 613 mg/l and 48h 471 mg/l.
Executive summary:

The study was perfomed according EU Method C.2 (Acute Toxicity for Daphnia).

The animals were treated and observed for 48 hours. No animal in the control group were immobilisized. The EC50 of Ethylcyanoacetat at 24 h is 613 mg/l and 48h 471 mg/l.