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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure according to national standards

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German industrial standard test guideline DIN 38 412, part L15.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): hydroxyethylpiperazine
- Analytical purity: 98 - 99%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The doses were chosen based on a range finding study.
The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 8.1 cm (range: 7.2 - 9.3)
- Weight at study initiation: 5.3 g (range: 3.6 - 8.3)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day


ACCLIMATION
- Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature: 20 ± 1°C
Acclimation to test water and test temperature: 3 days

- Type and amount of food: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum.

HOUSING
- Housing water: charcoal filtered water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Hardness: 2.5 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 8.0
- Water temperature: 15 - 20 °C
- Duration of housing: about 3 months
- Medical treatment: Twice with 0.05 mg/L Malachitegreen chloride and once with 10 mg/L tetracyclinhydrochlorid
- Feeding: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0.03 %

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
Total hardness: 2.5 mmol/L
Acid capacity: 0.8 mM
Test temperature:
20 - 21 °C
pH:
7.2 - 10.9
Dissolved oxygen:
7.2 - 8.9 mg/L
Nominal and measured concentrations:
Nominal concentrations: 100, 215, 464, 1000, 2150, 4640 mg/L. Additionally: Neutralised samples at 4640 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 5.3 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Na/K ratio: 10:1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

Reference substance (positive control):
yes
Remarks:
Chloracetamid

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 464 - < 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: apathy, narcotic-like state, tumbling


Results with reference substance (positive control):
- Results with reference substance valid? yes
- LC50: ca. 32 mg/L (48 h)

Any other information on results incl. tables

Cumulative mortality

Nominal conc.(mg/l)

Number of fish

Mortality (cumulated) after

1 h

4 h

24 h

48 h

72 h

96 h

0

10

0

0

0

0

0

0

100

10

0

0

0

0

0

0

215

10

0

0

0

0

0

0

464

10

0

0

0

0

0

0

1000

10

0

0

0

5

8

8

2150

10

0

3

10

10

10

10

4640

10

7

10

10

10

10

10

4640*

10

0

0

0

0

0

0

* = pH adjusted

observed symptoms

Nominal conc.(mg/l)

Symptoms after

1 h

4 h

24 h

48 h

72 h

96 h

0

 

 

 

 

 

 

100

 

 

 

 

 

 

215

 

 

 

 

 

 

464

 

 

 

 

 

 

1000

 

 

AT

A

A

A

2150

A

ANT

 

 

 

 

4640

N

 

 

 

 

 

4640*

 

 

 

 

 

 

*: pH-adjusted; A: Apathy, N: Narcotic like state, T: Tumbling

pH values

Nominal conc.(mg/l)

pH values after

1 h

24 h

48 h

72 h

96 h

0

8.2

8.0

7.7

7.6

7.7

100

9.4

8.2

7.7

7.7

7.7

215

9.7

9.0

7.8

7.7

7.7

464

10.0

9.3

8.3

8.0

8.0

1000

10.3

9.7

8.3

8.8

8.7

2150

10.6

10.2

 

 

 

4640

10.9

 

 

 

 

4640*

8.0

7.8

7.2

7.3

7.3

*: pH-adjusted

oxygen content

Nominal conc.(mg/l)

Oxygen concentrations [mg O2/L] after

1 h

24 h

48 h

72 h

96 h

0

7.9

7.4

8.0

7.3

7.1

100

7.7

8.2

8.2

7.8

7.5

215

8.2

7.2

8.6

8.9

8.5

464

8.3

7.7

8.8

8.7

8.4

1000

8.4

8.2

7.8

7.9

8.3

2150

8.6

8.8

 

 

 

4640

8.8

 

 

 

 

4640*

8.6

7.7

7.7

8.3

8.9

*: pH adjusted

Applicant's summary and conclusion

Validity criteria fulfilled:
yes