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One long-term study conducted with the earthworm Eisenia fetida, according to the OECD Guideline 222, is available for the subcategory 1 member Fatty acids, C16-18 and C18-unsaturated, branched and linear, “Monomer acid” (CAS No. 68955-98-6). 

As no effects occurred in the chronic earthworm study with Fatty acids, C16-18 and C18-unsaturated, branched and linear, “Monomer acid” (CAS No. 68955-98-6) up to the highest tested concentration of 100 mg/kg soil dw,

the members of the subcategory 1 predominantly monomers, are considered as not harmful to terrestrial organisms and therefore no further testing is needed.

As the test substance is not classified as toxic or harmful, therefore the substance is not expected to cause toxic effects in predators. Additionally, the uptake and bioaccumulation potential is low and thus the test substance is considered to cause no hazard to predators. Thus, a study with birds is not needed due to animal welfare reasons and no PNEC oral is derived from data on mammalian toxicity.