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Ecotoxicological information

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Long-term toxicity earthworm

Effects of Pigment Red 112 on mortality, biomass and the reproductive potential of the earthworm species Eisenia fetida were determined according to the guidelines DIN ISO 11268-2 (1998), OECD 222 (2004) and BBA (1994). The study was conducted under static conditions over 8 weeks with a limit test item concentration of 1000 mg test item/kg soil dry weight.

After 28 days no evident mortality as well as no pathological symptoms or changes in the behaviour of adult earthworms were seen in the controls as well as at the limit test item concentration of 1000 mg test item/kg soil dry weight.

The biomass increase of the adult earthworms after 28 days was not statistically significantly reduced compared to the pooled control at the limit test item concentration of 1000 mg test item/kg soil dry weight.

After eight weeks of exposure the earthworm reproduction was not statistically significantly reduced.

Overall, the NOEC of the test item concerning mortality, biomass and reproduction of earthworms was determined to be at 1000 mg test item/kg soil dry weight.

Toxicity to plants

The phytotoxicity of the test item Pigment Red 112 to three terrestrial plant species was determined in a seedling emergence test over a period of 21 days according to OECD 208. The test was conducted with the nominal test item concentration1000 mg/kg soil dry weight. Test systems were one monocotyledon, oats (Gramineae), and two dicotyledons, rape (Brassicaceae) and soybean (Leguminosae).

The toxic effects of the test item were determined on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and on day 21 by shoot height and shoot fresh weight determination. The inhibition of shoot height, shoot fresh weight and rate of emergence was calculated in relation to the untreated control.

The test item did not cause any effects > 25 % on the shoot height, the shoot fresh weight and the rate of emergence of the tested plant species at the tested concentration. After 21 days no test item related phytotoxic effects were observed for the tested plant species.

The test item Pigment Red 112 is not toxic at concentration ≤ 1000 mg/kg soil dry weight to the tested plant species.