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Toxicity to microorganisms

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toxicity to microorganisms, other
bacterial growth of Pseudomonas putida
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Principles of method if other than guideline:
Method: other: Bringmann and Kühn (1977), Z. Wasser Abwasser Forsch. 10,
GLP compliance:
Analytical monitoring:
Details on test solutions:
- Stock solution: 5.00 g test substance/l (were made up with bidistilled water ); gave pH = 11.5, which was neutralized with 10 % hydrochloric acid.
-Test concentrations ( 500 mg/L; 1000 mg/L ; 2000 mg/L and 4000 mg/L) were prepared by diluting the stock solution with bidestilled water.
Test organisms (species):
Pseudomonas putida
Test type:
Water media type:
Limit test:
Total exposure duration:
18 h
Remarks on exposure duration:
+/- 1h
Test temperature:
25 +/- 2 degree C
7.1 (Test 1); 7.0 (Test 2)
Nominal and measured concentrations:
Nominal: control / 500 / 1,000 / 2,000 / 4,000 mg/l
Details on test conditions:
- Number of replicates: 5 growth controls 4 controls 3 replicates of 4 concentrations all performed twice
- Test vessel: 250 ml Erlenmeyer flasks, sterile, capped with cellulose
- Duration of test: 18 +/- 1 hours
- Quantification: photometric determination of turbidity at 436 nm, graphical evaluation
Reference substance (positive control):
not specified
Key result
18 h
Dose descriptor:
Effect conc.:
1 120 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
other: extinction/absorption

EC10 = 1200 mg/l (Test 1)
1040 mg/l (Test 2);
mean = 1120 mg/l

Validity criteria fulfilled:
not specified
The EC10 (18 hours) was determined as 1120 mg/L indicating that the test substance does not pose a significant hazard to aquatic microorganism
Executive summary:

In a bacterial toxicity test using Pseudomonas putida (Hüls AG, 1996b) an 18 h-EC10 of 1,120 mg/l was obtained. Four concentrations ranging from 500 to 4000 mg/L were tested. The study was assessed as "reliable with restrictions".

Description of key information

In a bacterial toxicity test using Pseudomonas putida (Hüls AG, 1996b) an 18 h-EC10 of 1120 mg/l was obtained.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
1 120 mg/L

Additional information

18 h-EC10 value of 1120 mg/l