Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The 48-hr EC50 for Daphnia magna was determined to be 3.4 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
3.4 mg/L

Marine water invertebrates

Marine water invertebrates
Effect concentration:
2.4 mg/L

Additional information

Reliable (Klimisch 1 or 2) short-term toxicity studies with invertebrates have been conducted for o-tert-butylphenol or the structural analogue, o -sec-butylphenol and are included in this dossier.

Schulz (1995) conducted a reliable (Klimisch 2) GLP compliant study according to OECD 202 methods. The 48-hr acute toxicity of o-tert-butylphenol to Daphnia magna was studied under static conditions.  Daphnids were exposed to control and test chemical at nominal concentrations of 0.3, 0.6, 1.3, 2.5, 5.0, and 10 mg/L for 48 hr.  Stability of the test substance over the study period was confirmed by photometric analysis. Immobilisation and sublethal effects were observed daily.  The 48-hour EC50was reported to be 3.4 mg/L. 

McLeese et al (1981) conducted a reliable (Klimisch 2) toxicity study using o-tert-butylphenol.

The 96-hr acute toxicity of o-tert-butylphenol to the shrimp,Crangon septemspinosa, was studied under static conditions in saltwater.  Shrimp were exposed to control and test chemical at nominal concentrations for 96 hr.  The 96-hour LC50was reported to be 2.4 mg/L, based on mortality. 

MOE Japan (1999) conducted a reliable (Klimisch 2) GLP compliant study according to OECD 202 methods. The 48-hr acute toxicity of the structural analogue, o-sec-butylphenol, to Daphnia magna was studied under static conditions.  Daphnids were exposed to control and test chemical at nominal concentrations of 1.0, 1.8, 3.2, 5.6, and 10 mg/l for 48 hr.  Immobilisation was observed at 48 hours.  The 48-hour EC50was reported to be 4.0 mg/L. 

McLeese et al (1981) conducted a reliable (Klimisch 2) toxicity study using the structural analogue. The 96-hr acute toxicity of o-sec-butylphenol to the shrimp,Crangon septemspinosa, was studied under static conditions in saltwater.  Shrimp were exposed to control and test chemical at nominal concentrations for 96 hr.  The 96-hour LC50was reported to be 1.3 mg/L, based on mortality.

Reliable (Klimisch 2) short-term toxicity studies with invertebrates have been conducted for o-tert-butylphenol or the structural analogue, o -sec-butylphenol

and are included in this dossier. The key study, Schulz (1995), provides a 48-hr EC50 of 3.4 mg/L for o-tert-butylphenol for Daphnia magna. Additionally, McLeese et al (1981) provides a 96 -hr EC50 of 2.4 mg/L for o-tert-butylphenol for the saltwater shrimp. Consequently, these values will be taken as the short-term toxicity in aquatic invertebrates endpoint for o-tert-butylphenol.